ArticleBiophysical journal2001
The voltage-dependent proton pumping in bacteriorhodopsin is characterized by optoelectric behavior.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Driving forces of proton-pumping rhodopsins.Biophysical journal · 2024Article
- Improved production of β-carotene in light-powered Escherichia coli by co-expression of Gloeobacter rhodopsin expression.Microbial cell factories · 2023Article
- Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway.ACS physical chemistry Au · 2023Review
- Article
- Rhodopsin-Based Optogenetics: Basics and Applications.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Robust Photoelectric Biomolecular Switch at a Microcavity-Supported Lipid Bilayer.ACS applied materials & interfaces · 2021Article
- Article
- Engineered Passive Potassium Conductance in the KR2 Sodium Pump.Biophysical journal · 2019Article
- Functional characterization of sodium-pumping rhodopsins with different pumping properties.PloS one · 2017Article
- Conversion of a light-driven proton pump into a light-gated ion channel.Scientific reports · 2015Article
- The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination.Scientific reports · 2015Article
- Structure-guided transformation of channelrhodopsin into a light-activated chloride channel.Science (New York, N.Y.) · 2014Article
- Stable closure of the cytoplasmic half-channel is required for efficient proton transport at physiological membrane potentials in the bacteriorhodopsin catalytic cycle.Biochemistry · 2014Article
- Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin.Journal of the American Chemical Society · 2014Article
- Gloeobacter rhodopsin, limitation of proton pumping at high electrochemical load.Biophysical journal · 2013Article
- Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Voltage dependence of proton pumping by bacteriorhodopsin mutants with altered lifetime of the M intermediate.PloS one · 2013Article
- Article
- A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins.Nature methods · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The light-driven proton pump bacteriorhodopsin (bR) was functionally expressed in Xenopus laevis oocytes and in HEK-293 cells. The latter expression system allowed high time resolution of light-induced current signals. A detailed voltage clamp and patch clamp study was performed to investigate the DeltapH versus Deltapsi dependence of the pump current. The following results were obtained. The current voltage behavior of bR is linear in the measurable range between -160 mV and +60 mV. The pH dependence is less than expected from thermodynamic principles, i.e., one DeltapH unit produces a shift of the apparent reversal potential of 34 mV (and not 58 mV). The M(2)-BR decay shows a significant voltage dependence with time constants changing from 20 ms at +60 mV to 80 ms at -160 mV. The linear I-V curve can be reconstructed by this behavior. However, the slope of the decay rate shows a weaker voltage dependence than the stationary photocurrent, indicating that an additional process must be involved in the voltage dependence of the pump. A slowly decaying M intermediate (decay time > 100 ms) could already be detected at zero voltage by electrical and spectroscopic means. In effect, bR shows optoelectric behavior. The long-lived M can be transferred into the active photocycle by depolarizing voltage pulses. This is experimentally demonstrated by a distinct charge displacement. From the results we conclude that the transport cycle of bR branches via a long-lived M(1)* in a voltage-dependent manner into a nontransporting cycle, where the proton release and uptake occur on the extracellular side.
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