ArticleBiophysical journal2001
Reduced protein diffusion rate by cytoskeleton in vegetative and polarized dictyostelium cells.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 132 citations in OpenAlex.
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- Diversity in emergent cell locomotion from the coupling cytosolic and cortical Marangoni flows with reaction-diffusion dynamics.PLoS computational biology · 2026Article
- Cytoplasmic crowding acts as a porous medium reducing macromolecule diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Glycogen phase-separation drives macromolecular rearrangement and asymmetric division in E. coli.The EMBO journal · 2025Article
- Comparative Analysis of Biochemical and Cellular Assay Conditions and the Need for a Buffer That Mimics Cytoplasmic Environments.Molecules (Basel, Switzerland) · 2025Review
- Advection versus diffusion in brain ventricular transport.Fluids and barriers of the CNS · 2025Article
- Glycogen phase separation drives macromolecular rearrangement and asymmetric division inbioRxiv : the preprint server for biology · 2025Article
- Pair correlation microscopy of intracellular molecular transport.Nature protocols · 2025Review
- A Simple Method to Determine Diffusion Coefficients in Soft Hydrogels for Drug Delivery and Biomedical Applications.ACS omega · 2025Article
- From the Cytoplasm into the Nucleus-Hepatitis B Virus Travel and Genome Repair.Microorganisms · 2025Review
- Advanced multiparametric image spectroscopy and super-resolution microscopy reveal a minimal model of CD95 signal initiation.Science advances · 2024Article
- Intracellular Macromolecular Crowding within Individual Stress Fibers Analyzed by Fluorescence Correlation Spectroscopy.Cellular and molecular bioengineering · 2024Article
- Fluorescence Correlation Spectroscopy as a Versatile Method to Define Aptamer-Protein Interactions with Single-Molecule Sensitivity.Analytical chemistry · 2024Article
- Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations.Biophysical journal · 2023Article
- Mechanistic aspects of IPTG (isopropylthio-β-galactoside) transport across the cytoplasmic membrane of Escherichia coli-a rate limiting step in the induction of recombinant protein expression.Journal of industrial microbiology & biotechnology · 2023Article
- Actin filaments form a size-dependent diffusion barrier around centrosomes.EMBO reports · 2023Article
- Transport between im/mobile fractions shapes the speed and profile of cargo distribution in neurons.Biophysical reports · 2022Article
- Dependence of diffusion ineLife · 2022Article
- Hydrogels as functional components in artificial cell systems.Nature reviews. Chemistry · 2022Review
- Protein turnover dynamics suggest a diffusion-to-capture mechanism for peri-basal body recruitment and retention of intraflagellar transport proteins.Molecular biology of the cell · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence recovery after photobleaching measurements with high spatial resolution are performed to elucidate the impact of the actin cytoskeleton on translational mobility of green fluorescent protein (GFP) in aqueous domains of Dictyostelium discoideum amoebae. In vegetative Dictyostelium cells, GFP molecules experience a 3.6-fold reduction of their translational mobility relative to dilute aqueous solutions. In disrupting the actin filamentous network using latrunculin-A, the intact actin cytoskeletal network is shown to contribute an effective viscosity of 1.36 cP, which accounts for 53% of the restrained molecular diffusion of GFP. The remaining 47% of hindered protein motions is ascribed to other mechanical barriers and the viscosity of the cell liquid. A direct correlation between the density of the actin network and its limiting action on protein diffusion is furthermore established from measurements under different osmotic conditions. In highly locomotive polarized cells, the obstructing effect of the actin filamentous network is seen to decline to 0.46 cP in the non-cortical regions of the cell. Our results indicate that the meshwork of actin filaments constitutes the primary mechanical barrier for protein diffusion and that any noticeable reorganization of the network is accompanied by altered intracellular protein mobility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.