ArticleRNA (New York, N.Y.)2001
The ATP requirement for U2 snRNP addition is linked to the pre-mRNA region 5' to the branch site.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Branch site recognition by the spliceosome.RNA (New York, N.Y.) · 2024Review
- Article
- A model for DHX15 mediated disassembly of A-complex spliceosomes.RNA (New York, N.Y.) · 2022Article
- Structural basis of branch site recognition by the human spliceosome.Science (New York, N.Y.) · 2022Article
- Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing.Nature structural & molecular biology · 2013Article
- The anti-tumor drug E7107 reveals an essential role for SF3b in remodeling U2 snRNP to expose the branch point-binding region.Genes & development · 2011Article
- Effect of ATP binding and hydrolysis on dynamics of canine parvovirus NS1.Journal of virology · 2010Article
- Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing.RNA (New York, N.Y.) · 2010Article
- RS domains contact splicing signals and promote splicing by a common mechanism in yeast through humans.Genes & development · 2006Article
- Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA.The EMBO journal · 2004Article
- Immunopurified small nucleolar ribonucleoprotein particles pseudouridylate rRNA independently of their association with phosphorylated Nopp140.Molecular and cellular biology · 2002Article
- hnRNP A1 controls HIV-1 mRNA splicing through cooperative binding to intron and exon splicing silencers in the context of a conserved secondary structure.RNA (New York, N.Y.) · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Association of U2 snRNP with the pre-mRNA branch region is the first ATP-dependent step in spliceosome assembly. The basis of this energy dependence is not known. Previously, we identified minimal intron-derived substrates that form complexes with U2 independent of ATP. Here, we identify the intron region linked to the ATP dependence of this step by comparing these substrates to longer RNAs that recapitulate the ATP requirement. This region needed to impose ATP dependence lies immediately 5' to the branch site. Sequences ranging from 6 to 14 nt yield a near linear inhibitory effect on efficiency of complex formation with U2 snRNP, with 18 nt yielding near maximal ATP dependence. This region is not protected prior to U2 addition, and RNase H targeting of the region within nuclear extract converts an ATP-dependent substrate into an ATP-independent one. Within this region, there is no sequence specificity linked with the ATP requirement, as neither a specific sequence is needed, nor even nucleobases. These data and the results of other modifications suggest models in which the 18-nt region is a target for interactions with U2 snRNP in an ATP-bound or -activated conformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.