ArticleRNA (New York, N.Y.)2001
Modulation of msl-2 5' splice site recognition by Sex-lethal.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Genome-Wide Identification and Characterization of UTR-Introns ofInternational journal of molecular sciences · 2020Article
- Hrp48 and eIF3d contribute to msl-2 mRNA translational repression.Nucleic acids research · 2018Article
- Article
- Promiscuity in post-transcriptional control of gene expression: Drosophila sex-lethal and its regulatory partnerships.FEBS letters · 2017Review
- The expanding universe of ribonucleoproteins: of novel RNA-binding proteins and unconventional interactions.Pflugers Archiv : European journal of physiology · 2016Review
- Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle.The Journal of biological chemistry · 2013Article
- Sex-lethal promotes nuclear retention of msl2 mRNA via interactions with the STAR protein HOW.Genes & development · 2013Article
- Before It Gets Started: Regulating Translation at the 5' UTR.Comparative and functional genomics · 2012Article
- Regulated functional alternative splicing in Drosophila.Nucleic acids research · 2012Review
- The translation initiation factor eIF4E regulates the sex-specific expression of the master switch gene Sxl in Drosophila melanogaster.PLoS genetics · 2011Article
- Distinct regulatory programs establish widespread sex-specific alternative splicing in Drosophila melanogaster.RNA (New York, N.Y.) · 2011Article
- iCLIP predicts the dual splicing effects of TIA-RNA interactions.PLoS biology · 2010Article
- Heterogeneous nuclear ribonucleoprotein K represses the production of pro-apoptotic Bcl-xS splice isoform.The Journal of biological chemistry · 2009Article
- X chromosomal regulation in flies: when less is more.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- RNA binding protein sex-lethal (Sxl) and control of Drosophila sex determination and dosage compensation.Microbiology and molecular biology reviews : MMBR · 2003Review
- U1 snRNP-dependent function of TIAR in the regulation of alternative RNA processing of the human calcitonin/CGRP pre-mRNA.Molecular and cellular biology · 2003Article
- U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5' splice sites.The Biochemical journal · 2003Article
- Sex lethal and U2 small nuclear ribonucleoprotein auxiliary factor (U2AF65) recognize polypyrimidine tracts using multiple modes of binding.RNA (New York, N.Y.) · 2003Article
- The splicing regulator TIA-1 interacts with U1-C to promote U1 snRNP recruitment to 5' splice sites.The EMBO journal · 2002Article
- Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein Sex-lethal (SXL) controls dosage compensation in Drosophila by inhibiting splicing and subsequently translation of male-specific-lethal-2 (msl-2) transcripts. We have previously shown that SXL blocks the binding of U2 auxiliary factor (U2AF) to the polypyrimidine (Py)-tract associated with the 3' splice site of the regulated intron. We now report that a second pyrimidine-rich sequence containing 11 consecutive uridines immediately downstream from the 5' splice site is required for efficient splicing inhibition by SXL. Psoralen-mediated crosslinking experiments suggest that SXL binding to this uridine-rich sequence inhibits recognition of the 5' splice site by U1 snRNP in HeLa nuclear extracts. We also show that SXL interferes with the binding of the protein TIA-1 to the uridine-rich stretch. Because TIA-1 binding to this sequence is necessary for U1 snRNP recruitment to msl-25' splice site and for splicing of this pre-mRNA, we propose that SXL antagonizes TIA-1 activity and thus prevents 5' splice site recognition by U1 snRNP. Taken together with previous data, we conclude that efficient retention of msl-2 intron involves inhibition of early recognition of both splice sites by SXL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.