ArticleMolecular and cellular biology2001
Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 112 citations in OpenAlex.
- DNA lesions can frequently precede DNA:RNA hybrid accumulation.Nature communications · 2025Article
- A R-loop sensing pathway mediates the relocation of transcribed genes to nuclear pore complexes.Nature communications · 2023Article
- The THO complex counteracts TERRA R-loop-mediated telomere fragility in telomerase+ cells and telomeric recombination in ALT+ cells.Nucleic acids research · 2023Article
- Native functions of short tandem repeats.eLife · 2023Article
- Spt4 facilitates the movement of RNA polymerase II through the +2 nucleosomal barrier.Cell reports · 2021Article
- Sir3 mediates long-range chromosome interactions in budding yeast.Genome research · 2021Article
- R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast.Molecular biology of the cell · 2021Article
- Structural insights into the nucleic acid remodeling mechanisms of the yeast THO-Sub2 complex.eLife · 2020Article
- Harmful DNA:RNA hybrids are formedeLife · 2020Article
- Mip6 binds directly to the Mex67 UBA domain to maintain low levels of Msn2/4 stress-dependent mRNAs.EMBO reports · 2019Article
- The THO Complex Coordinates Transcripts for Synapse Development and Dopamine Neuron Survival.Cell · 2018Article
- Regulation of transcription elongation in response to osmostress.PLoS genetics · 2017Article
- Depletion of three combined THOC5 mRNA export protein target genes synergistically induces human hepatocellular carcinoma cell death.Oncogene · 2016Article
- Splicing-independent loading of TREX on nascent RNA is required for efficient expression of dual-strand piRNA clusters in Drosophila.Genes & development · 2016Article
- The Transcription Factor THO Promotes Transcription Initiation and Elongation by RNA Polymerase I.The Journal of biological chemistry · 2016Article
- Nuclear pore components affect distinct stages of intron-containing gene expression.Nucleic acids research · 2015Article
- THOC5 controls 3'end-processing of immediate early genes via interaction with polyadenylation specific factor 100 (CPSF100).Nucleic acids research · 2014Article
- Genome-wide profiling of yeast DNA:RNA hybrid prone sites with DRIP-chip.PLoS genetics · 2014Article
- THOC5, a member of the mRNA export complex: a novel link between mRNA export machinery and signal transduction pathways in cell proliferation and differentiation.Cell communication and signaling : CCS · 2014Review
- Structural characterization of the Saccharomyces cerevisiae THO complex by small-angle X-ray scattering.PloS one · 2014Article
4 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Hpr1 forms, together with Tho2, Mft1, and Thp2, the THO complex, which controls transcription elongation and genome stability in Saccharomyces cerevisiae. Mutations in genes encoding the THO complex confer strong transcription-impairment and hyperrecombination phenotypes in the bacterial lacZ gene. In this work we demonstrate that Hpr1 is a factor required for transcription of long as well as G+C-rich DNA sequences. Using different lacZ segments fused to the GAL1 promoter, we show that the negative effect of lacZ sequences on transcription depends on their distance from the promoter. In parallel, we show that transcription of either a long LYS2 fragment or the S. cerevisiae YAT1 G+C-rich open reading frame fused to the GAL1 promoter is severely impaired in hpr1 mutants, whereas transcription of LAC4, the Kluyveromyces lactis ortholog of lacZ but with a lower G+C content, is only slightly affected. The hyperrecombination behavior of the DNA sequences studied is consistent with the transcriptional defects observed in hpr1 cells. These results indicate that both length and G+C content are important elements influencing transcription in vivo. We discuss their relevance for the understanding of the functional role of Hpr1 and, by extension, the THO complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.