ArticleMolecular and cellular biology2001
MSY2 and MSY4 bind a conserved sequence in the 3' untranslated region of protamine 1 mRNA in vitro and in vivo.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 85 citations in OpenAlex.
- Y-box binding proteins in immunity and RNA virus infection.Microbiology and molecular biology reviews : MMBR · 2025Review
- TTC36-Mediated Tumor Suppression via YBX3/SPRED1 Axis Paradoxically Reduces Sorafenib Sensitivity in Hepatocellular Carcinoma.International journal of biological sciences · 2025Article
- Silencing circSERPINE2 restrains mesenchymal stem cell senescence via the YBX3/PCNA/p21 axis.Cellular and molecular life sciences : CMLS · 2023Article
- Expanding the binding specificity for RNA recognition by a PUF domain.Nature communications · 2021Article
- Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality.Cell and tissue research · 2021Article
- Review
- Y-Box Binding Proteins in mRNP Assembly, Translation, and Stability Control.Biomolecules · 2020Review
- Compound Heterozygosity for Y Box Proteins Causes Sterility Due to Loss of Translational Repression.PLoS genetics · 2015Article
- The role of cold shock domain proteins in inflammatory diseases.Journal of molecular medicine (Berlin, Germany) · 2014Review
- Stress- and Rho-activated ZO-1-associated nucleic acid binding protein binding to p21 mRNA mediates stabilization, translation, and cell survival.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Bioinformatic identification of novel elements potentially involved in messenger RNA fate control during spermatogenesis.Biology of reproduction · 2012Article
- Cold-shock-domain protein A (CSDA) contributes posttranscriptionally to gonadotropin-releasing hormone-regulated expression of Egr1 and indirectly to Lhb.Biology of reproduction · 2012Article
- The Nanos3-3'UTR is required for germ cell specific NANOS3 expression in mouse embryos.PloS one · 2010Article
- Cold shock domain family members YB-1 and MSY4 share essential functions during murine embryogenesis.Molecular and cellular biology · 2006Article
- MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
- Absence of the DNA-/RNA-binding protein MSY2 results in male and female infertility.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- YB-1 autoregulates translation of its own mRNA at or prior to the step of 40S ribosomal subunit joining.Molecular and cellular biology · 2005Article
- The DNA/RNA-binding protein MSY2 marks specific transcripts for cytoplasmic storage in mouse male germ cells.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A walk though vertebrate and invertebrate protamines.Chromosoma · 2003Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Y-box proteins are major constituents of ribonucleoprotein particles (RNPs) which contain translationally silent mRNAs in gametic cells. We have recently shown that a sequence-specific RNA binding activity present in spermatogenic cells contains the two Y-box proteins MSY2 and MSY4. We show here that MSY2 and MSY4 bind a sequence, 5'-UCCAUCA-3', present in the 3' untranslated region of the translationally repressed protamine 1 (Prm1) mRNA. Using pre- and post-RNase T1-digested substrate RNAs, it was determined that MSY2 and MSY4 can bind an RNA of eight nucleotides containing the MSY2 and MSY4 binding site. Single nucleotide mutations in the sequence eliminated the binding of MSY2 and MSY4 in an electrophoretic mobility shift assay, and the resulting mutants failed to compete for binding in a competition assay. A consensus site of U(AC)C(A)CAU(C)CA(CU) (subscripts indicate nucleotides which do not disrupt YRS binding by MSY2 and MSY4), denoted the Y-box recognition site (YRS), was defined from this mutational analysis. These mutations in the YRS were further characterized in vivo using a novel application of the yeast three-hybrid system. Experiments with transgenic mice show that disruption of the YRS in vivo relieves Prm1-like repression of a reporter gene. The conservation of the RNA binding motifs among Y-box protein family members raises the possibility that other Y-box proteins may have previously unrecognized sequence-specific RNA binding activities.
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