ArticleMolecular and cellular biology2001
Membrane raft-dependent regulation of phospholipase Cgamma-1 activation in T lymphocytes.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- Phosphoinositide acyl chain saturation drives CD8Nature immunology · 2023Article
- Innate immunity orchestrates the mobilization and homing of hematopoietic stem/progenitor cells by engaging purinergic signaling-an update.Purinergic signalling · 2020Review
- Noncatalytic Bruton's tyrosine kinase activates PLCγThe Journal of biological chemistry · 2020Article
- Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells.The EMBO journal · 2011Article
- LKB1 regulates TCR-mediated PLCγ1 activation and thymocyte positive selection.The EMBO journal · 2011Article
- Article
- Polyphosphoinositides are enriched in plant membrane rafts and form microdomains in the plasma membrane.Plant physiology · 2010Article
- Role of nonreceptor protein tyrosine kinases during phospholipase C-gamma 1-related uterine contractions in the rat.Reproductive sciences (Thousand Oaks, Calif.) · 2009Article
- Tec kinases regulate T-lymphocyte development and function: new insights into the roles of Itk and Rlk/Txk.Immunological reviews · 2009Review
- Lipid rafts and T-lymphocyte function: implications for autoimmunity.FEBS letters · 2008Review
- Cholera toxin, LT-I, LT-IIa and LT-IIb: the critical role of ganglioside binding in immunomodulation by type I and type II heat-labile enterotoxins.Expert review of vaccines · 2007Review
- Dynamic recruitment of phospholipase C gamma at transiently immobilized GPI-anchored receptor clusters induces IP3-Ca2+ signaling: single-molecule tracking study 2.The Journal of cell biology · 2007Article
- A critical role for the E3-ligase activity of c-Cbl in VEGFR-2-mediated PLCgamma1 activation and angiogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Intramolecular regulation of phospholipase C-gamma1 by its C-terminal Src homology 2 domain.Molecular and cellular biology · 2007Article
- Lipid rafts and regulation of the cytoskeleton during T cell activation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2005Review
- Regulation of T-cell receptor signalling by membrane microdomains.Immunology · 2004Review
- Requirements for distinct steps of phospholipase Cgamma2 regulation, membrane-raft-dependent targeting and subsequent enzyme activation in B-cell signalling.The Biochemical journal · 2003Article
- Article
- 70Z/3 Cbl induces PLC gamma 1 activation in T lymphocytes via an alternate Lat- and Slp-76-independent signaling mechanism.Oncogene · 2003Article
- MEK kinase 2 and the adaptor protein Lad regulate extracellular signal-regulated kinase 5 activation by epidermal growth factor via Src.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Numerous signaling molecules associate with lipid rafts, either constitutively or after engagement of surface receptors. One such molecule, phospholipase Cgamma-1 (PLCgamma1), translocates from the cytosol to lipid rafts during T-cell receptor (TCR) signaling. To investigate the role played by lipid rafts in the activation of this molecule in T cells, an influenza virus hemagglutinin A (HA)-tagged PLCgamma1 was ectopically expressed in Jurkat T cells and targeted to these microdomains by the addition of a dual-acylation signal. Raft-targeted PLCgamma1 was constitutively tyrosine phosphorylated and induced constitutive NF-AT-dependent transcription and interleukin-2 secretion in Jurkat cells. Tyrosine phosphorylation of raft-targeted PLCgamma1 did not require Zap-70 or the interaction with the adapters Lat and Slp-76, molecules that are necessary for TCR signaling. In contrast, the Src family kinase Lck was required. Coexpression in HEK 293T cells of PLCgamma1-HA with Lck or the Tec family kinase Rlk resulted in preferential phosphorylation of raft-targeted PLCgamma1 over wild-type PLCgamma1. These data show that localization of PLCgamma1 in lipid rafts is sufficient for its activation and demonstrate a role for lipid rafts as microdomains that dynamically segregate and integrate PLCgamma1 with other signaling components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.