ArticleMolecular and cellular biology2001
Asymmetric processing of human immunodeficiency virus type 1 cDNA in vivo: implications for functional end coupling during the chemical steps of DNA transposition.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 44 citations in OpenAlex.
- Differential assembly of Rous sarcoma virus tetrameric and octameric intasomes is regulated by the C-terminal domain and tail region of integrase.The Journal of biological chemistry · 2018Article
- Multifunctional facets of retrovirus integrase.World journal of biological chemistry · 2015Review
- Structural dynamics of native and V260E mutant C-terminal domain of HIV-1 integrase.Journal of computer-aided molecular design · 2015Article
- Uneven genetic robustness of HIV-1 integrase.Journal of virology · 2015Article
- Quantitative analysis of the time-course of viral DNA forms during the HIV-1 life cycle.Retrovirology · 2013Article
- A homology model of HIV-1 integrase and analysis of mutations designed to test the model.Journal of molecular biology · 2013Article
- Differential effects of human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity of viral DNA integration.Journal of virology · 2013Article
- Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus.Journal of virology · 2012Article
- HIV-1 integrase strand transfer inhibitors stabilize an integrase-single blunt-ended DNA complex.Journal of molecular biology · 2011Article
- HIV DNA is heavily uracilated, which protects it from autointegration.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Structure-based modeling of the functional HIV-1 intasome and its inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Functional disruption of the moloney murine leukemia virus preintegration complex by vaccinia-related kinases.The Journal of biological chemistry · 2010Article
- Article
- Flexible use of nuclear import pathways by HIV-1.Cell host & microbe · 2010Article
- Molecular Interactions between HIV-1 integrase and the two viral DNA ends within the synaptic complex that mediates concerted integration.Journal of molecular biology · 2009Article
- Biochemical and biophysical analyses of concerted (U5/U3) integration.Methods (San Diego, Calif.) · 2009Article
- HIV-1 Integrase Strand Transfer Inhibitors: Novel Insights into their Mechanism of Action.Retrovirology : research and treatment · 2008Article
- Mechanisms of human immunodeficiency virus type 1 concerted integration related to strand transfer inhibition and drug resistance.Antimicrobial agents and chemotherapy · 2008Article
- Inhibition of human immunodeficiency virus type 1 concerted integration by strand transfer inhibitors which recognize a transient structural intermediate.Journal of virology · 2007Article
- Transcriptional coactivator LEDGF/p75 modulates human immunodeficiency virus type 1 integrase-mediated concerted integration.Journal of virology · 2007Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Retroviral integration, like all forms of DNA transposition, proceeds through a series of DNA cutting and joining reactions. During transposition, the 3' ends of linear transposon or donor DNA are joined to the 5' phosphates of a double-stranded cut in target DNA. Single-end transposition must be avoided in vivo because such aberrant DNA products would be unstable and the transposon would therefore risk being lost from the cell. To avoid suicidal single-end integration, transposons link the activity of their transposase protein to the combined functionalities of both donor DNA ends. Although previous work suggested that this critical coupling between transposase activity and DNA ends occurred before the initial hydrolysis step of retroviral integration, work in the related Tn10 and V(D)J recombination systems had shown that end coupling regulated transposase activity after the initial hydrolysis step of DNA transposition. Here, we show that integrase efficiently hydrolyzed just the wild-type end of two different single-end mutants of human immunodeficiency virus type 1 in vivo, which, in contrast to previous results, proves that two functional DNA ends are not required to activate integrase's initial hydrolysis activity. Furthermore, despite containing bound protein at their processed DNA ends, these mutant viruses did not efficiently integrate their singly cleaved wild-type end into target DNA in vitro. By comparing our results to those of related DNA recombination systems, we propose the universal model that end coupling regulates transposase activity after the first chemical step of DNA transposition.
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