ArticleThe Journal of cell biology2001
Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Pathophysiological role of ion channels and transporters in gastrointestinal mucosal diseases.Cellular and molecular life sciences : CMLS · 2021Review
- Omeprazole preserves the RER in chief cells and enhances re-epithelialization of parietal cells with SOD and AQP-4 up-regulation in ethanol-induced gastritis rats.Experimental and therapeutic medicine · 2017Article
- Regulation and Function of AQP4 in the Central Nervous System.Neurochemical research · 2015Review
- H3 receptor renal expression in normal and diabetic rats.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2015Article
- Article
- Live cell analysis of aquaporin-4 m1/m23 interactions and regulated orthogonal array assembly in glial cells.The Journal of biological chemistry · 2009Article
- Article
- Carnosine protects against Abeta42-induced neurotoxicity in differentiated rat PC12 cells.Cellular and molecular neurobiology · 2008Article
- Concerted vs. sequential. Two activation patterns of vast arrays of intracellular Ca2+ channels in muscle.The Journal of general physiology · 2005Article
- What are aquaporins for?The Journal of membrane biology · 2004Review
- The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Partitioning of aquaporin-4 water channel mRNA and protein in gastric glands.Digestive diseases and sciences · 2003Article
- Polarized trafficking and surface expression of the AQP4 water channel are coordinated by serial and regulated interactions with different clathrin-adaptor complexes.The EMBO journal · 2001Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To test the involvement of the water channel aquaporin (AQP)-4 in gastric acid physiology, the human gastric cell line (HGT)-1 was stably transfected with rat AQP4. AQP4 was immunolocalized to the basolateral membrane of transfected HGT-1 cells, like in native parietal cells. Expression of AQP4 in transfected cells increased the osmotic water permeability coefficient (Pf) from 2.02 +/- 0.3 x 10-4 to 16.37 +/- 0.5 x 10-4 cm/s at 20 degrees C. Freeze-fracture EM showed distinct orthogonal arrays of particles (OAPs), the morphological signature of AQP4, on the plasma membrane of AQP4-expressing cells. Quantitative morphometry showed that the density of OAPs was 2.5 +/- 0.3% under basal condition and decreased by 50% to 1.2 +/- 0.3% after 20 min of histamine stimulation, mainly due to a significant decrease of the OAPs number. Concomitantly, Pf decreased by approximately 35% in 20-min histamine-stimulated cells. Both Pf and OAPs density were not modified after 10 min of histamine exposure, time at which the maximal hormonal response is observed. Cell surface biotinylation experiments confirmed that AQP4 is internalized after 20 min of histamine exposure, which may account for the downregulation of water transport. This is the first evidence for short term rearrangement of OAPs in an established AQP4-expressing cell line.
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