ArticleThe Journal of cell biology2001
Essential control of an endothelial cell ISOC by the spectrin membrane skeleton.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Critical role of alpha spectrin in DNA repair: the importance of μ-calpain and Fanconi anemia proteins.Experimental biology and medicine (Maywood, N.J.) · 2025Review
- The Molecular Heterogeneity of Store-Operated CaInternational journal of molecular sciences · 2023Review
- Role of Spectrin in Endocytosis.Cells · 2022Review
- Noncanonical function of long myosin light chain kinase in increasing ER-PM junctions and augmentation of SOCE.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- S100A6 is a positive regulator of PPP5C-FKBP51-dependent regulation of endothelial calcium signaling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- The Spectrinome: The Interactome of a Scaffold Protein Creating Nuclear and Cytoplasmic Connectivity and Function.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- The potential role of spectrin network in the mechanotransduction of MLO-Y4 osteocytes.Scientific reports · 2017Article
- Spectrin's chimeric E2/E3 enzymatic activity.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Sodium entry through endothelial store-operated calcium entry channels: regulation by Orai1.American journal of physiology. Cell physiology · 2015Article
- Article
- Mechanisms regulating endothelial permeability.Pulmonary circulation · 2014Review
- The serine-threonine phosphatase calcineurin is a regulator of endothelial store-operated calcium entry.Pulmonary circulation · 2014Article
- Regulation of store-operated calcium entry by FK506-binding immunophilins.Cell calcium · 2013Article
- Orai1 determines calcium selectivity of an endogenous TRPC heterotetramer channel.Circulation research · 2012Article
- Spectrin-based skeleton as an actor in cell signaling.Cellular and molecular life sciences : CMLS · 2012Review
- Cytoskeleton reorganization as an alternative mechanism of store-operated calcium entry control in neuroendocrine-differentiated cells.PloS one · 2012Article
- TRPing on the lung endothelium: calcium channels that regulate barrier function.Antioxidants & redox signaling · 2009Review
- Physiology and pathophysiology of canonical transient receptor potential channels.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Review
- The actin cytoskeleton in endothelial cell phenotypes.Microvascular research · 2009Review
- Cav3.1 (alpha1G) controls von Willebrand factor secretion in rat pulmonary microvascular endothelial cells.American journal of physiology. Lung cellular and molecular physiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mechanism(s) underlying activation of store-operated Ca2+ entry currents, ISOC, remain incompletely understood. F-actin configuration is an important determinant of channel function, although the nature of interaction between the cytoskeleton and ISOC channels is unknown. We examined whether the spectrin membrane skeleton couples Ca2+ store depletion to Ca2+ entry. Thapsigargin activated an endothelial cell ISOC (-45 pA at -80 mV) that reversed at +40 mV, was inwardly rectifying when Ca2+ was the charge carrier, and was inhibited by La3+ (50 microM). Disruption of the spectrin-protein 4.1 interaction at residues A207-V445 of betaSpIISigma1 decreased the thapsigargin-induced global cytosolic Ca2+ response by 50% and selectively abolished the endothelial cell ISOC, without altering activation of a nonselective current through cyclic nucleotide-gated channels. In contrast, disruption of the spectrin-actin interaction at residues A47-K186 of betaSpIISigma1 did not decrease the thapsigargin-induced global cytosolic Ca2+ response or inhibit ISOC. Results indicate that the spectrin-protein 4.1 interaction selectively controls ISOC, indicating that physical coupling between calcium release and calcium entry is reliant upon the spectrin membrane skeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.