ArticleThe Journal of cell biology2001
Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 160 citations in OpenAlex.
- Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20.British journal of cancer · 2024Article
- Therapeutic gene silencing ofScience advances · 2023Article
- Review
- Mechanisms by Which Kinesin-5 Motors Perform Their Multiple Intracellular Functions.International journal of molecular sciences · 2021Review
- HSP70 regulates Eg5 distribution within the mitotic spindle and modulates the cytotoxicity of Eg5 inhibitors.Cell death & disease · 2020Article
- Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans.PLoS genetics · 2019Article
- Recent synthetic and medicinal perspectives of dihydropyrimidinones: A review.European journal of medicinal chemistry · 2017Review
- Cooperation Between Kinesin Motors Promotes Spindle Symmetry and Chromosome Organization in Oocytes.Genetics · 2017Article
- A comprehensive model to predict mitotic division in budding yeasts.Molecular biology of the cell · 2015Article
- Impact of kinesin Eg5 inhibition by 3,4-dihydropyrimidin-2(1H)-one derivatives on various breast cancer cell features.BMC cancer · 2015Article
- A computational model for the formation of lamin-B mitotic spindle envelope and matrix.Interface focus · 2014Article
- Small molecule screen for candidate antimalarials targeting Plasmodium Kinesin-5.The Journal of biological chemistry · 2014Article
- Review
- Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface.The Journal of cell biology · 2013Article
- The bipolar assembly domain of the mitotic motor kinesin-5.Nature communications · 2013Article
- Chlamydial infection induces host cytokinesis failure at abscission.Cellular microbiology · 2012Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts.Nature protocols · 2012Article
- Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2.Molecular biology of the cell · 2012Article
- The molecular basis of anaphase A in animal cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We used fluorescent speckle microscopy to probe the dynamics of the mitotic kinesin Eg5 in Xenopus extract spindles, and compared them to microtubule dynamics. We found significant populations of Eg5 that were static over several seconds while microtubules flux towards spindle poles. Eg5 dynamics are frozen by adenylimidodiphosphate. Bulk turnover experiments showed that Eg5 can exchange between the spindle and the extract with a half life of <55 s. Eg5 distribution in spindles was not perturbed by inhibition of its motor activity with monastrol, but was perturbed by inhibition of dynactin with p50 dynamitin. We interpret these data as revealing the existence of a static spindle matrix that promotes Eg5 targeting to spindles, and transient immobilization of Eg5 within spindles. We discuss alternative interpretations of the Eg5 dynamics we observe, ideas for the biochemical nature of a spindle matrix, and implications for Eg5 function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.