ArticleEnvironmental health perspectives2001
Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (Danio rerio).
Article in Environmental health perspectives, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 64 citations in OpenAlex.
- Discovery of a novel AhR-CYP1A1 axis activator for mitigating inflammatory diseases using anActa pharmaceutica Sinica. B · 2025Article
- Development, scrutiny, and modulation of transient reporter gene assays of the xenobiotic metabolism pathway in zebrafish hepatocytes.Cell biology and toxicology · 2023Article
- More than Meets the Eye: The Aryl Hydrocarbon Receptor is an Environmental Sensor, Physiological Regulator and a Therapeutic Target in Ocular Disease.Frontiers in toxicology · 2022Review
- Zebrafish in Toxicology and Environmental Health.Current topics in developmental biology · 2017Review
- Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution.Transgenic research · 2016Article
- Zebrafish: A marvel of high-throughput biology for 21Current environmental health reports · 2014Article
- Article
- Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos.BMC biotechnology · 2012Article
- Nitro-fatty acids and cyclopentenone prostaglandins share strategies to activate the Keap1-Nrf2 system: a study using green fluorescent protein transgenic zebrafish.Genes to cells : devoted to molecular & cellular mechanisms · 2011Article
- Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation.Ecotoxicology (London, England) · 2010Article
- Estrogen-responsive transient expression assay using a brain aromatase-based reporter gene in zebrafish (Danio rerio).Comparative medicine · 2009Article
- Perturbation of defense pathways by low-dose arsenic exposure in zebrafish embryos.Environmental health perspectives · 2009Article
- The zebrafish embryo model in environmental risk assessment--applications beyond acute toxicity testing.Environmental science and pollution research international · 2008Review
- Direct, continuous monitoring of air pollution by transgenic sensor mice responsive to halogenated and polycyclic aromatic hydrocarbons.Environmental health perspectives · 2008Article
- Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line.Environmental health perspectives · 2002Article
- The state of the art of the zebrafish model for toxicology and toxicologic pathology research--advantages and current limitations.Toxicologic pathologyReview
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3 authors at 1 institution in 1 country.
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Abstract
We developed an inducible in vivo reporter system to examine expression of the aryl hydrocarbon receptor (AhR) during development in zebrafish (Danio rerio). AhR is a ligand-activated transcription factor that mediates the toxic actions of environmental contaminants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Induction of cytochrome P4501A1 (CYP1A1) is an early biomarker of AhR activation. A 1905 base pair region of the human CYP1A1 promoter/enhancer region was regulated by AhR in zebrafish liver cells after exposure to TCDD (10 nM) in a transient transfection assay. This regulatory region was fused to the cDNA sequence encoding green fluorescent protein (GFP) of jellyfish (Aequorea victoria). Transgenic zebrafish were generated to express this AhR-regulated GFP construct. Injected fish exposed to TCDD exhibited induction of GFP in the eye, nose, and vertebrae of zebrafish embryos (48 and 72 hr after fertilization) compared to vehicle controls (DMSO), which did not express GFP. To investigate whether AhR-regulated GFP expression correlated with sites of TCDD toxicity, we exposed wild-type zebrafish to DMSO or TCDD and examined them for morphologic abnormalities. By 5 days after fertilization, TCDD-exposed fish exhibited gross dysmorphogenesis in cranio-facial and vertebral development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.