ArticleProceedings of the National Academy of Sciences of the United States of America2001
Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 124 citations in OpenAlex.
- TheBiomolecules · 2025Review
- Diversity of sialic acids and sialoglycoproteins in gametes and at fertilization.Frontiers in cell and developmental biology · 2022Review
- Article
- In Silico Reconstruction of Sperm Chemotaxis.International journal of molecular sciences · 2021Article
- The role of female reproductive fluid in sperm competition.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Tracing the Evolutionary History of the CAP Superfamily of Proteins Using Amino Acid Sequence Homology and Conservation of Splice Sites.Journal of molecular evolution · 2017Article
- Natriuretic peptide type C induces sperm attraction for fertilization in mouse.Scientific reports · 2017Article
- A Novel Cysteine Knot Protein for Enhancing Sperm Motility That Might Facilitate the Evolution of Internal Fertilization in Amphibians.PloS one · 2016Article
- Dicalcin, a zona pellucida protein that regulates fertilization competence of the egg coat in Xenopus laevis.The journal of physiological sciences : JPS · 2015Review
- CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization.The Journal of cell biology · 2015Article
- Accelerated evolution of schistosome genes coding for proteins located at the host-parasite interface.Genome biology and evolution · 2015Article
- Transmembrane signal transduction in oocyte maturation and fertilization: focusing on Xenopus laevis as a model animal.International journal of molecular sciences · 2014Review
- Subcellular metabolite and lipid analysis of Xenopus laevis eggs by LAESI mass spectrometry.PloS one · 2014Article
- Article
- Cryptic choice of conspecific sperm controlled by the impact of ovarian fluid on sperm swimming behavior.Evolution; international journal of organic evolution · 2013Article
- Temporal sampling, resetting, and adaptation orchestrate gradient sensing in sperm.The Journal of cell biology · 2012Article
- Expression of odorant receptor family, type 2 OR in the aquatic olfactory cavity of amphibian frog Xenopus tropicalis.PloS one · 2012Article
- Two types of assays for detecting frog sperm chemoattraction.Journal of visualized experiments : JoVE · 2011Article
- Sperm chemotaxis, fluid shear, and the evolution of sexual reproduction.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Sperm and oocyte communication mechanisms controlling C. elegans fertility.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we demonstrated that a protein from Xenopus egg jelly exhibits sperm chemoattractant activity when assayed by either video microscopy or by sperm passage across a porous filter. Here we describe the isolation and purification of allurin, the protein responsible for this activity. Freshly oviposited jellied eggs were soaked in buffer, and the conditioned medium was loaded onto an anion exchange column and eluted with an NaCl gradient. The active fraction was purified further by RP-HPLC, the chemoattractant protein appearing as a single sharp peak. The amino acid sequence of the protein, determined by direct sequencing and cloning of cDNAs coding for the protein, consisted of 184 amino acids having a molecular mass of 21,073 Da. The protein shares homology with the mammalian cysteine-rich secretory protein (CRISP) family that includes testes-specific spermatocyte protein 1, a cell adhesion protein which links spermatocytes to Seritoli cells, and acidic epididymal glycoproteins that bind to sperm and have been implicated in sperm-egg fusion. Phylogenetic analysis suggests that allurin evolved from the ancestral protein that gave rise to the mammalian CRISP family. Addition of allurin to this family portends that the CRISP family represents a group of "sperm escort" proteins, which bind to sperm at various steps in their life history, facilitating passage from one functional stage to the next. Allurin stands out in this regard, representing both the first vertebrate sperm chemoattractant to be purified and sequenced and the first member of the CRISP family to be found in the female reproductive tract.
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