ArticleProceedings of the National Academy of Sciences of the United States of America2001
In vivo transposition of Minos, a Drosophila mobile element, in mammalian tissues.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature.Bioresources and bioprocessing · 2022Article
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- A native, highly active Tc1/mariner transposon from zebrafish (ZB) offers an efficient genetic manipulation tool for vertebrates.Nucleic acids research · 2021Article
- Establishment of transgenic lines for jumpstarter method using a composite transposon vector in the ladybird beetle, Harmonia axyridis.PloS one · 2014Article
- Regulation of DNA transposition by CpG methylation and chromatin structure in human cells.Mobile DNA · 2013Article
- Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells.Nature protocols · 2011Article
- Article
- DNA transposons: nature and applications in genomics.Current genomics · 2010Article
- Tagged mutagenesis by efficient Minos-based germ line transposition.Molecular and cellular biology · 2010Article
- Development of a database system for mapping insertional mutations onto the mouse genome with large-scale experimental data.BMC genomics · 2009Article
- Cancer gene discovery in mouse and man.Biochimica et biophysica acta · 2009Review
- Whole-body sleeping beauty mutagenesis can cause penetrant leukemia/lymphoma and rare high-grade glioma without associated embryonic lethality.Cancer research · 2009Article
- The NemaGENETAG initiative: large scale transposon insertion gene-tagging in Caenorhabditis elegans.Genetica · 2009Review
- Transposon-mediated genome manipulation in vertebrates.Nature methods · 2009Review
- Transposon tools hopping in vertebrates.Briefings in functional genomics & proteomics · 2008Review
- PBmice: an integrated database system of piggyBac (PB) insertional mutations and their characterizations in mice.Nucleic acids research · 2008Article
- Post-integration behavior of a Minos transposon in the malaria mosquito Anopheles stephensi.Molecular genetics and genomics : MGG · 2007Article
- The DNA transposon Minos as a tool for transgenesis and functional genomic analysis in vertebrates and invertebrates.Genome biology · 2007Review
- Transposons for cancer gene discovery: Sleeping Beauty and beyond.Genome biology · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Drosophila hydei, to transpose in mouse tissues. Two transgenic mouse lines were crossed, one expressing Minos transposase in lymphocytes under the control of the CD2 promoter/locus control region and another carrying a nonautonomous Minos transposon. Only mice containing both transgenes show excision of the transposon and transposition into new chromosomal sites in thymus and spleen cells. In addition, expression of Minos transposase in embryonic fibroblast cell lines derived from a transposon-carrying transgenic mouse resulted in excision of the transposon. These results are a first step toward a reversible insertional mutagenesis system in the mouse, opening the way to develop powerful technologies for functional genomic analysis in mammals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.