ArticleProceedings of the National Academy of Sciences of the United States of America2001
In vivo interactions of archaeal Cdc6/Orc1 and minichromosome maintenance proteins with the replication origin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 135 citations in OpenAlex.
- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
- The Archaeal Cell Cycle.Annual review of cell and developmental biology · 2024Review
- Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dissection of Functional Domains of Orc1-2, the Archaeal Global DNA Damage-Responsive Regulator.International journal of molecular sciences · 2022Article
- Micrococcal Nuclease Digestion Assays for the Analysis of Chromosome Structure in Archaea.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Compendium of Methods to Uncover RNA-Protein Interactions In Vivo.Methods and protocols · 2021Review
- G-Quadruplexes in the Archaea Domain.Biomolecules · 2020Article
- Archaeal DNA Replication.Annual review of microbiology · 2020Review
- Emerging views of genome organization in Archaea.Journal of cell science · 2020Review
- Origins of DNA replication.PLoS genetics · 2019Review
- Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging.Nucleic acids research · 2018Article
- Do Archaea Need an Origin of Replication?Trends in microbiology · 2018Review
- Genome Replication inFrontiers in microbiology · 2017Article
- Comparative genomics reveals conserved positioning of essential genomic clusters in highly rearranged Thermococcales chromosomes.Biochimie · 2015Article
- Activation of a dormant replication origin is essential for Haloferax mediterranei lacking the primary origins.Nature communications · 2015Article
- Choosing a suitable method for the identification of replication origins in microbial genomes.Frontiers in microbiology · 2015Review
- Association between the dynamics of multiple replication origins and the evolution of multireplicon genome architecture in haloarchaea.Genome biology and evolution · 2014Article
- Multiple replication origins with diverse control mechanisms in Haloarcula hispanica.Nucleic acids research · 2014Article
- Archaeal chromosome biology.Journal of molecular microbiology and biotechnology · 2014Review
- DNA replication origins in archaea.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although genome analyses have suggested parallels between archaeal and eukaryotic replication systems, little is known about the DNA replication mechanism in Archaea. By two-dimensional gel electrophoreses we positioned a replication origin (oriC) within 1 kb in the chromosomal DNA of Pyrococcus abyssi, an anaerobic hyperthermophile, and demonstrated that the oriC is physically linked to the cdc6 gene. Our chromatin immunoprecipitation assays indicated that P. abyssi Cdc6 and minichromosome maintenance (MCM) proteins bind preferentially to the oriC region in the exponentially growing cells. Whereas the oriC association of MCM was specifically inhibited by stopping DNA replication with puromycin treatment, Cdc6 protein stayed bound to the replication origin after de novo protein synthesis was inhibited. Our data suggest that archaeal and eukaryotic Cdc6 and MCM proteins function similarly in replication initiation and imply that an oriC association of MCM could be regulated by an unknown mechanism in Archaea.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.