ArticleGenetics2001
Technical knockout, a Drosophila model of mitochondrial deafness.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 99 citations in OpenAlex.
- The First Report of a Bang-Sensitive Strain inInternational journal of molecular sciences · 2026Article
- Absence of Mother's Curse for performance traits among divergent mtDNAs in heterozygous nuclear backgrounds in Drosophila.bioRxiv : the preprint server for biology · 2025Article
- A naturally occurring mitochondrial genome variant confers broad protection from infection in Drosophila.PLoS genetics · 2024Article
- Noncoupled Mitochondrial Respiration as Therapeutic Approach for the Treatment of Metabolic Diseases: Focus on Transgenic Animal Models.International journal of molecular sciences · 2023Review
- Nuclear genetic background influences the phenotype of the Drosophila tko25t mitochondrial protein-synthesis mutant.G3 (Bethesda, Md.) · 2023Article
- Mitochondrial Neurodegeneration: Lessons fromBiomolecules · 2023Review
- Our current understanding of the toxicity of altered mito-ribosomal fidelity during mitochondrial protein synthesis: What can it tell us about human disease?Frontiers in physiology · 2023Review
- Mitochondria Dysfunction in Frontotemporal Dementia/Amyotrophic Lateral Sclerosis: Lessons FromFrontiers in neuroscience · 2021Review
- Mitochondrial Protein Translation: Emerging Roles and Clinical Significance in Disease.Frontiers in cell and developmental biology · 2021Review
- Regulation of growth in Drosophila melanogaster: the roles of mitochondrial metabolism.Journal of biochemistry · 2020Review
- Review
- MRPS16 facilitates tumor progression via the PI3K/AKT/Snail signaling axis.Journal of Cancer · 2020Article
- Groove structure of porous hydroxyapatite scaffolds (HAS) modulates immune environment via regulating macrophages and subsequently enhances osteogenesis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2019Article
- Minimal effects ofBiology open · 2019Article
- Lethal Interaction of Nuclear and Mitochondrial Genotypes inG3 (Bethesda, Md.) · 2019Article
- Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Shortened Lifespan and Other Age-Related Defects in Bang Sensitive Mutants ofG3 (Bethesda, Md.) · 2018Article
- Developmental arrest in Drosophila melanogaster caused by mitochondrial DNA replication defects cannot be rescued by the alternative oxidase.Scientific reports · 2018Article
- Nutritional Interventions for Mitochondrial OXPHOS Deficiencies: Mechanisms and Model Systems.Annual review of pathology · 2018Review
- Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutations in mtDNA-encoded components of the mitochondrial translational apparatus are associated with diverse pathological states in humans, notably sensorineural deafness. To develop animal models of such disorders, we have manipulated the nuclear gene for mitochondrial ribosomal protein S12 in Drosophila (technical knockout, tko). The prototypic mutant tko(25t) exhibits developmental delay, bang sensitivity, impaired male courtship, and defective response to sound. On the basis of a transgenic reversion test, these phenotypes are attributable to a single substitution (L85H) at a conserved residue of the tko protein. The mutant is hypersensitive to doxycyclin, an antibiotic that selectively inhibits mitochondrial protein synthesis, and mutant larvae have greatly diminished activities of mitochondrial redox enzymes and decreased levels of mitochondrial small-subunit rRNA. A second mutation in the tko gene, Q116K, which is predicted to impair the accuracy of mitochondrial translation, results in the completely different phenotype of recessive female sterility, based on three independent transgenic insertions. We infer that the tko(25t) mutant provides a model of mitochondrial hearing impairment resulting from a quantitative deficiency of mitochondrial translational capacity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.