ArticleGenetics2001
TCA1, a single nuclear-encoded translational activator specific for petA mRNA in Chlamydomonas reinhardtii chloroplast.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025Review
- Phylogeny and evolutionary dynamics of the Rubia genus based on the chloroplast genome of Rubia tibetica.Scientific reports · 2025Article
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- The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo inThe Plant cell · 2020Article
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.Nucleic acids research · 2017Article
- Spontaneous dominant mutations in chlamydomonas highlight ongoing evolution by gene diversification.The Plant cell · 2015Article
- A nucleus-encoded chloroplast protein regulated by iron availability governs expression of the photosystem I subunit PsaA in Chlamydomonas reinhardtii.Plant physiology · 2015Article
- Cytosine deaminase as a negative selectable marker for the microalgal chloroplast: a strategy for the isolation of nuclear mutations that affect chloroplast gene expression.The Plant journal : for cell and molecular biology · 2014Article
- The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins.World journal of microbiology & biotechnology · 2014Review
- Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii.The Plant cell · 2014Article
- A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas.Plant molecular biology · 2012Article
- Stable plastid transformation for high-level recombinant protein expression: promises and challenges.Journal of biomedicine & biotechnology · 2012Article
- Mutagenesis and phenotypic selection as a strategy toward domestication of Chlamydomonas reinhardtii strains for improved performance in photobioreactors.Photosynthesis research · 2011Article
- The nucleus-encoded trans-acting factor MCA1 plays a critical role in the regulation of cytochrome f synthesis in Chlamydomonas chloroplasts.The Plant cell · 2011Article
- Molecular identification and function of cis- and trans-acting determinants for petA transcript stability in Chlamydomonas reinhardtii chloroplasts.Molecular and cellular biology · 2008Article
- Evidence for regulatory function of nucleus-encoded factors on mRNA stabilization and translation in the chloroplast.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Defects in the cytochrome b6/f complex prevent light-induced expression of nuclear genes involved in chlorophyll biosynthesis.Plant physiology · 2006Article
- Biogenesis of PSI involves a cascade of translational autoregulation in the chloroplast of Chlamydomonas.The EMBO journal · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated seven allelic nuclear mutants of Chlamydomonas reinhardtii specifically blocked in the translation of cytochrome f, a major chloroplast-encoded subunit of the photosynthetic electron transport chain encoded by the petA gene. We recovered one chloroplast suppressor in which the coding region of petA was now expressed under the control of a duplicated 5' untranslated region from another open reading frame of presently unknown function. Since we also recovered 14 nuclear intragenic suppressors, we ended up with 21 alleles of a single nuclear gene we called TCA1 for translation of cytochrome b(6)f complex petA mRNA. The high number of TCA1 alleles, together with the absence of genetic evidence for other nuclear loci controlling translation of the chloroplast petA gene, strongly suggests that TCA1 is the only trans-acting factor. We studied the assembly-dependent regulation of cytochrome f translation--known as the CES process--in TCA1-mutated contexts. In the presence of a leaky tca1 allele, we observed that the regulation of cytochrome f translation was now exerted within the limits of the restricted translational activation conferred by the altered version of TCA1 as predicted if TCA1 was the ternary effector involved in the CES process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.