ArticleJournal of virology2001
Transient disruption of intercellular junctions enables baculovirus entry into nondividing hepatocytes.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Improving promiscuous mammalian cell entry by the baculovirus Autographa californica multiple nuclear polyhedrosis virus.Bioscience reports · 2012Article
- Recombinant baculovirus as a highly potent vector for gene therapy of human colorectal carcinoma: molecular cloning, expression, and in vitro characterization.Molecular biotechnology · 2010Article
- Baculovirus vectors for gene therapy.Advances in virus research · 2006Review
- Baculovirus entry into human hepatoma cells.Journal of virology · 2005Article
- Baculovirus as versatile vectors for protein expression in insect and mammalian cells.Nature biotechnology · 2005Review
- Baculovirus as a highly efficient expression vector in insect and mammalian cells.Acta pharmacologica Sinica · 2005Review
- Improvement in nuclear entry and transgene expression of baculoviruses by disintegration of microtubules in human hepatocytes.Journal of virology · 2005Article
- Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes.Gene therapy · 2003Article
- Expression of E-cadherin and other paracellular junction genes is decreased in iron-loaded hepatocytes.The American journal of pathology · 2003Article
- Identification and characterization of Hyphantria cunea nucleopolyhedrovirus homologous repeated regions.Virus genes · 2002Article
- Effects of electroporation on primary rat hepatocytes in vitro.World journal of gastroenterology · 2002Article
- Rebound of hepatitis B virus replication in HepG2 cells after cessation of antiviral treatment.Journal of virology · 2002Article
- Gene transfer mediated by recombinant baculovirus into mouse eye.Investigative ophthalmology & visual science · 2001Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Baculovirus infection has extended the capabilities for transfection of exogenous genes into a variety of mammalian cell types. Because rat hepatocytes plated on collagen-coated dishes and maintained in dimethyl sulfoxide (DMSO)-supplemented chemically defined medium are an excellent model system for studying liver function in vitro, we investigated the ability of baculoviruses to infect and deliver exogenous genes to cells in this culture system. Efficient delivery to hepatocytes in short-term culture becomes restricted to peripheral cells, or "edge" cells, as the hepatocytes acquire intercellular junctions and form islands with time in culture. This barrier to baculovirus entry can be overcome, and the percentage of internal cells within the hepatocyte islands that are infected with the baculovirus can be increased more than 100-fold, when cells are subjected to transient calcium depletion before and during infection. These findings suggest that at least in some cell types, such as hepatocytes, baculovirus entry may require contact with the basolateral surface. We conclude from this study that recombinant baculovirus infection following transient depletion of extracellular calcium results in delivery of exogenous genes to at least 75% of hepatocytes in long-term DMSO culture, thereby making it possible for the first time to carry out gain-of-function and loss-of-function studies in this cell system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.