ArticleJournal of virology2001
Overexpression of simian virus 40 small-T antigen blocks centrosome function and mitotic progression in human fibroblasts.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- JC virus small tumor antigen promotes S phase entry and cell cycle progression.Tumour virus research · 2024Article
- Microtubules in Polyomavirus Infection.Viruses · 2020Review
- Microtubule Regulation and Function during Virus Infection.Journal of virology · 2017Review
- Article
- Immunodetection of SV40 T/t-antigens in human osteosrcoma in a series of Tunisian patients.Pathology oncology research : POR · 2012Article
- Requirements for protein phosphorylation and the kinase activity of polo-like kinase 1 (Plk1) for the kinetochore function of mitotic arrest deficiency protein 1 (Mad1).The Journal of biological chemistry · 2008Article
- PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen.Oncogene · 2008Article
- Dephosphorylation of JC virus agnoprotein by protein phosphatase 2A: inhibition by small t antigen.Virology · 2008Article
- Small tumor antigen of polyomaviruses: role in viral life cycle and cell transformation.Journal of cellular physiology · 2008Review
- Simian virus 40 in humans.Infectious agents and cancer · 2007Article
- Epstein-Barr virus thymidine kinase is a centrosomal resident precisely localized to the periphery of centrioles.Journal of virology · 2007Article
- HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomes.Molecular biology of the cell · 2004Article
- Emerging roles of DNA tumor viruses in cell proliferation: new insights into genomic instability.Oncogene · 2003Review
- Simian virus 40 infection of humans.Journal of virology · 2003Article
- Brain tumors and polyomaviruses.Journal of neurovirology · 2003Review
- Simian virus 40 small tumor antigen induces deregulation of the actin cytoskeleton and tight junctions in kidney epithelial cells.Journal of virology · 2003Article
- Negative regulation of mitotic promoting factor by the checkpoint kinase chk1 in simian virus 40 lytic infection.Journal of virology · 2003Article
- The Epstein-Barr virus immediate-early protein BZLF1 induces both a G(2) and a mitotic block.Journal of virology · 2002Article
- Centrosome amplification and the development of cancer.Oncogene · 2002Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Recombinant adenoviruses that express high levels of the simian virus 40 (SV40) small-t (ST) antigen have been used to study the requirement for ST to drive cell cycle proliferation of confluent human diploid fibroblasts. This occurs when either large-T (LT) antigen or serum is added to provide a second signal. While cells readily completed S phase in these experiments, they were found to accumulate with 4N DNA content. Cellular and nuclear morphology, as well as the biochemical status of cyclin B complexes, showed that these cells entered mitosis but were blocked prior to mitotic metaphase. The defect appears to reflect an inability of cells overexpressing ST to form organized centrosomes that duplicate and separate normally during the cell cycle and, therefore, the absence of a mitotic spindle. The ability of ST to bind protein phosphatase 2A was required for this pattern, suggesting that altered phosphorylation of key centrosomal components may occur when ST is overexpressed. Although the possible significance of ST effects on the centrosome cycle is not fully understood, these findings suggest that ST could influence chromosomal instability patterns that are a hallmark of SV40-transformed cells and LT expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.