ArticleEMBO reports2001
Novel critical role of a human Mediator complex for basal RNA polymerase II transcription.
Article in EMBO reports, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 122 citations in OpenAlex.
- Transcriptomic Signatures of Immune Suppression and Cellular Dysfunction Distinguish Latent from Transcriptionally Active HIV-1 Infection in Dendritic Cells.International journal of molecular sciences · 2026Article
- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- Article
- Targeting transcriptional regulators for treatment of anaplastic thyroid cancer.Journal of cancer metastasis and treatment · 2021Article
- Reconstitution of Pol II (G) responsive form of the human Mediator complex.Turkish journal of biology = Turk biyoloji dergisi · 2021Article
- PPARβ/δ recruits NCOR and regulates transcription reinitiation of ANGPTL4.Nucleic acids research · 2019Article
- Smad7:β-catenin complex regulates myogenic gene transcription.Cell death & disease · 2019Article
- The Role of Tripartite Motif Family Proteins in TGF-β Signaling Pathway and Cancer.Journal of cancer prevention · 2018Review
- Article
- Clinical, pathologic, cytogenetic, and molecular profiling in self-identified black women with uterine leiomyomata.Cancer genetics · 2018Article
- Cardiac Med1 deletion promotes early lethality, cardiac remodeling, and transcriptional reprogramming.American journal of physiology. Heart and circulatory physiology · 2017Article
- PIC Activation through Functional Interplay between Mediator and TFIIH.Journal of molecular biology · 2017Article
- Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme.The Journal of biological chemistry · 2016Article
- The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum.Plant physiology · 2015Article
- Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants.Frontiers in plant science · 2015Review
- Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.Nature structural & molecular biology · 2014Article
- A functional portrait of Med7 and the mediator complex in Candida albicans.PLoS genetics · 2014Article
- All-atomic molecular dynamic studies of human CDK8: insight into the A-loop, point mutations and binding with its partner CycC.Computational biology and chemistry · 2014Article
- Disordered proteinaceous machines.Chemical reviews · 2014Review
- Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.Cell · 2014Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human Mediator complexes have been described as important bridging factors that enhance the effect of activators in purified systems and in chromatin. Here we report a novel basal function of a human Mediator complex. A monoclonal antibody was generated that depleted the majority of Mediator components from crude cell extracts. The removal of human Mediator abolished transcription by RNA polymerase II. This was observed on all genes tested, on TATA-containing and TATA-less promoters, both in the presence and absence of activators. To identify the relevant complex a combined biochemical and immunopurification protocol was applied. Two variants termed Mediator and basal Mediator were functionally and structurally distinguished. Basal Mediator function relies on additional constraints, which is reflected in the observation that it is essential in crude but not in purified systems. We conclude that basal Mediator is a novel general transcription factor of RNA polymerase II.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.