ArticleNucleic acids research2001
A kinetic analysis of substrate recognition by uracil-DNA glycosylase from herpes simplex virus type 1.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Uracil-DNA glycosylase efficiency is modulated by substrate rigidity.Scientific reports · 2023Article
- Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening.Biochemistry and biophysics reports · 2021Article
- Specificity and Efficiency of the Uracil DNA Glycosylase-Mediated Strand Cleavage Surveyed on Large Sequence Libraries.Scientific reports · 2019Article
- N-terminal domain of human uracil DNA glycosylase (hUNG2) promotes targeting to uracil sites adjacent to ssDNA-dsDNA junctions.Nucleic acids research · 2018Article
- Detection of uracil within DNA using a sensitive labeling method for in vitro and cellular applications.Nucleic acids research · 2016Article
- Lesion search and recognition by thymine DNA glycosylase revealed by single molecule imaging.Nucleic acids research · 2015Article
- 7,8-Dihydro-8-oxoadenine, a highly mutagenic adduct, is repaired by Escherichia coli and human mismatch-specific uracil/thymine-DNA glycosylases.Nucleic acids research · 2013Article
- Structural basis for the recognition and cleavage of abasic DNA in Neisseria meningitidis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway.Nucleic acids research · 2011Article
- Solid state 2H NMR analysis of furanose ring dynamics in DNA containing uracil.The journal of physical chemistry. B · 2010Article
- AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis.The EMBO journal · 2007Article
- Different structural states in oligonucleosomes are required for early versus late steps of base excision repair.Nucleic acids research · 2007Article
- Uracil-DNA glycosylases SMUG1 and UNG2 coordinate the initial steps of base excision repair by distinct mechanisms.Nucleic acids research · 2007Article
- A rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping.Nucleic acids research · 2007Article
- Suppressed catalytic activity of base excision repair enzymes on rotationally positioned uracil in nucleosomes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Uracil in DNA--occurrence, consequences and repair.Oncogene · 2002Review
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uracil-DNA glycosylase (UDG) is responsible for the removal of uracil from DNA. It has previously been demonstrated that UDG exhibits some sequence dependence in its activity, although this has not been well characterised. This study has investigated the sequence-dependent activity of UDG from herpes simplex virus type-1 (HSV-1). A more detailed analysis has been possible by using both kinetic and binding assays with a variety of different oligonucleotide substrates. The target uracil has been placed in substrates with either A-T-rich or G-C-rich flanking sequences and analyses have been performed on both the single- and double-stranded forms of each substrate. In the latter the uracil has been placed in both a U.A base pair and a U.G mismatch. It is observed that the sequences flanking the target uracil have a greater effect on UDG activity than the partner base of the uracil. Furthermore, the sequence context effects extend to single-stranded DNA. Systematic examination of the kinetics and binding of UDG with these different substrates has enabled us to examine the origin of the sequence preferences. We conclude that the damage recognition step in the HSV-1 UDG reaction pathway is modulated by local DNA sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.