ArticleNucleic acids research2001
Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cells.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- SUMO control of centromere homeostasis.Frontiers in cell and developmental biology · 2023Review
- Ctf3/CENP-I provides a docking site for the desumoylase Ulp2 at the kinetochore.The Journal of cell biology · 2021Article
- Stable inheritance of CENP-A chromatin: Inner strength versus dynamic control.The Journal of cell biology · 2020Article
- Recruitment of the Ulp2 protease to the inner kinetochore prevents its hyper-sumoylation to ensure accurate chromosome segregation.PLoS genetics · 2019Article
- The SUMO Pathway in Mitosis.Advances in experimental medicine and biology · 2017Review
- Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.Nature communications · 2014Article
- The dimerization domain of PfCENP-C is required for its functions as a centromere protein in human malaria parasite Plasmodium falciparum.Malaria journal · 2014Article
- SENP1 and SENP2 affect spatial and temporal control of sumoylation in mitosis.Molecular biology of the cell · 2013Article
- Transcription in the maintenance of centromere chromatin identity.Nucleic acids research · 2012Review
- SUMOylation in control of accurate chromosome segregation during mitosis.Current protein & peptide science · 2012Review
- CENP-C recruits M18BP1 to centromeres to promote CENP-A chromatin assembly.The Journal of cell biology · 2011Article
- Centromeres: unique chromatin structures that drive chromosome segregation.Nature reviews. Molecular cell biology · 2011Review
- The fate of metaphase kinetochores is weighed in the balance of SUMOylation during S phase.Cell cycle (Georgetown, Tex.) · 2010Review
- Article
- Dual recognition of CENP-A nucleosomes is required for centromere assembly.The Journal of cell biology · 2010Article
- Dissection of CENP-C-directed centromere and kinetochore assembly.Molecular biology of the cell · 2009Article
- The C-terminal domain of CENP-C displays multiple and critical functions for mammalian centromere formation.PloS one · 2009Article
- Emerging roles of the SUMO pathway in mitosis.Cell division · 2008Article
- The role of SUMO in chromosome segregation.Chromosoma · 2007Review
- The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation.The Journal of cell biology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CENP-C is an evolutionarily conserved centromere protein that is thought to be an important component in kinetochore assembly in vertebrate cells. However, the functional role of CENP-C in cell cycle progression remains unclear. To further understand CENP-C function, we developed a method incorporating the hyper-recombinogenic chicken B lymphocyte cell line DT40 to create several temperature-sensitive CENP-C mutants in DT40 cells. We found that, under restrictive conditions, one temperature-sensitive mutant, ts4-11, displayed metaphase delay and chromosome missegregation but proceeded through the cell cycle until arrest at G(1) phase. Furthermore, ts4-11 cells were transfected with a human HeLa cell cDNA library maintained in a retroviral vector, and genes that suppressed the temperature-sensitive phenotype were identified. One of these suppressor genes encodes SUMO-1, which is a ubiquitin-like protein. This finding suggests that SUMO-1 may be involved in centromere function in vertebrate cells. The novel strategy reported here will be useful and applicable to a wide range of proteins that have general cell-autonomous function in vertebrate cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.