ArticleAntimicrobial agents and chemotherapy2001
Sequencing of cytomegalovirus UL97 gene for genotypic antiviral resistance testing.
Article in Antimicrobial agents and chemotherapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 95 citations in OpenAlex.
- Emergence of Letermovir-resistant HCMV UL56 mutant during rescue treatment in a liver transplant recipient with ganciclovir-resistant infection HCMV: a case report.BMC infectious diseases · 2021Article
- Prevalence of UL97 gene mutations and polymorphisms in cytomegalovirus infection in the colon associated with or without ulcerative colitis.Scientific reports · 2021Article
- The Cytomegalovirus Protein Kinase pUL97:Host Interactions, Regulatory Mechanisms and Antiviral Drug Targeting.Microorganisms · 2020Review
- How I treat resistant cytomegalovirus infection in hematopoietic cell transplantation recipients.Blood · 2016Article
- Analysis of ganciclovir-resistant human herpesvirus 6B clinical isolates using quenching probe PCR methodology.Antimicrobial agents and chemotherapy · 2015Article
- Differential properties of cytomegalovirus pUL97 kinase isoforms affect viral replication and maribavir susceptibility.Journal of virology · 2014Article
- Successful ganciclovir treatment of primary cytomegalovirus infection containing the UL97 mutation N510S in an intestinal graft recipient.Infection · 2013Article
- Rapid detection of human cytomegalovirus UL97 and UL54 mutations directly from patient samples.Journal of clinical microbiology · 2013Article
- Effects on maribavir susceptibility of cytomegalovirus UL97 kinase ATP binding region mutations detected after drug exposure in vitro and in vivo.Antiviral research · 2012Article
- Cytomegalovirus retinitis and the acquired immunodeficiency syndrome--bench to bedside: LXVII Edward Jackson Memorial Lecture.American journal of ophthalmology · 2011Article
- Does Cytomegalovirus Develop Resistance following Antiviral Prophylaxis and Treatment in Renal Transplant Patients in Kuwait?Advances in virology · 2011Article
- Antiviral drug resistance of human cytomegalovirus.Clinical microbiology reviews · 2010Review
- Stereoselective phosphorylation of cyclopropavir by pUL97 and competitive inhibition by maribavir.Antimicrobial agents and chemotherapy · 2010Article
- Recombinant phenotyping of cytomegalovirus UL97 kinase sequence variants for ganciclovir resistance.Antimicrobial agents and chemotherapy · 2010Article
- Lack of prompt expansion of cytomegalovirus pp65 and IE-1-specific IFNgamma CD8+ and CD4+ T cells is associated with rising levels of pp65 antigenemia and DNAemia during pre-emptive therapy in allogeneic hematopoietic stem cell transplant recipients.Bone marrow transplantation · 2010Article
- Successful valganciclovir treatment of post-transplant cytomegalovirus infection in the presence of UL97 mutation N597D.Journal of medical virology · 2009Article
- Rapid detection directly from patient serum samples of human cytomegalovirus UL97 mutations conferring ganciclovir resistance.Journal of clinical microbiology · 2007Article
- Multidrug resistance conferred by novel DNA polymerase mutations in human cytomegalovirus isolates.Antimicrobial agents and chemotherapy · 2007Article
- Detection of ganciclovir resistance in patients with AIDS and cytomegalovirus retinitis: correlation of genotypic methods with viral phenotype and clinical outcome.The Journal of infectious diseases · 2006Article
- Phenotyping of cytomegalovirus drug resistance mutations by using recombinant viruses incorporating a reporter gene.Antimicrobial agents and chemotherapy · 2005Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The widespread use of ganciclovir (GCV) to treat cytomegalovirus (CMV) infections in immunosuppressed patients has led to the development of drug resistance. Phenotypic assays for CMV drug resistance are presently too time-consuming to be therapeutically useful. To support the development of genotypic assays for GCV resistance, the complete sequences of the UL97 phosphotransferase genes in 28 phenotypically GCV-sensitive CMV clinical isolates were determined. The gene was found to be highly conserved, with nucleotide sequence identity among strains ranging from 98.6 to 100% and amino acid sequence identity of >99%. Primers for a genotypic assay were designed to amplify codons 400 to 707, because all known UL97 mutations conferring drug resistance occur at three sites within this region. This part of the UL97 gene was amplified from over 50 clinical isolates, and two sequencing reactions for the coding strand were successfully used to identify GCV resistance mutations. This genotypic assay can be performed in 48 h using genomic DNA extracted from cell monolayers at very low levels of virus infectivity, thus rapidly providing therapeutically useful results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.