ArticleJournal of anatomy2001
Immunolabelling, histochemistry and in situ hybridisation in human skeletal muscle fibres to detect myosin heavy chain expression at the protein and mRNA level.
Article in Journal of anatomy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Inverse relationship between exercise economy and oxidative capacity in muscle.European journal of applied physiology · 2005Trial
- Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence.International journal of molecular sciences · 2022Article
- Increased biological relevance of transcriptome analyses in human skeletal muscle using a model-specific pipeline.BMC bioinformatics · 2020Article
- Muscle Fiber Typing in Bovine and Porcine Skeletal Muscles Using Immunofluorescence with Monoclonal Antibodies Specific to Myosin Heavy Chain Isoforms.Food science of animal resources · 2020Article
- Label-free LC-MS/MS shotgun proteomics to investigate the anti-inflammatory effect of rCC16.Molecular medicine reports · 2016Article
- Differential expression of skeletal muscle genes following administration of clenbuterol to exercised horses.BMC genomics · 2016Article
- Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.Muscle & nerve · 2014Article
- Myosin heavy chain composition of the human genioglossus muscle.Journal of speech, language, and hearing research : JSLHR · 2012Article
- Article
- Preliminary observations of muscle fibre cross sectional area of flexor digitorum brevis in cadaver feet with and without claw toes.Journal of foot and ankle research · 2010Article
- Myosin heavy-chain composition of the human hyoglossus muscle.Dysphagia · 2010Article
- Sarcomeric myosin expression in the tongue body of humans, macaques and rats.Cells, tissues, organs · 2010Article
- A comprehensive characterisation of the fibre composition and properties of a limb (flexor digitorum superficialis, membri thoraci) and a trunk (psoas major) muscle in cattle.BMC cell biology · 2008Article
- Immunocytochemical characteristics of elbow, knee and ankle muscles of the five-toed jerboa (Allactaga elater).Journal of anatomy · 2003Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The distribution of muscle fibres classified on the basis of their content of different myosin heavy chain (MHC) isoforms was analysed in vastus lateralis muscle biopsies of 15 young men (with an average age of 22 y) by correlating immunohistochemistry with specific anti-MHC monoclonal antibodies, myofibrillar ATPase (mATPase) histochemistry and in situ hybridisation with probes specific for MHC beta-slow, MHC-IIA and MHC-IIX. The characterisation of a large number of individual fibres was compared and correlated on a fibre-to-fibre basis. The panel of monoclonal antibodies used in the study allowed classification of human skeletal muscle fibres into 5 categories according to the MHC isoform they express at the protein level, types I, I+IIA, IIA, IIAX and IIX. Hybrid fibres coexpressing two isoforms represented a considerable proportion of the fibre composition (about 14%) and were clearly underestimated by mATPase histochemistry. For a very high percentage of fibres there was a precise correspondence between the MHC protein isoforms and mRNA transcripts. The integrated methods used demonstrate a high degree of precision of the immunohistochemical procedure used for the identification and quantification of human skeletal muscle fibre types. The monoclonal antibody S5-8H2 is particularly useful for identifying hybrid IIAX fibres. This protocol offers new prospects for muscle fibre classification in human experimental studies.
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