ArticleProceedings of the National Academy of Sciences of the United States of America2001
Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 82 citations in OpenAlex.
- Transgenesis in Drug Discovery: Enhancing Target Identification and Validation.Molecular biotechnology · 2026Review
- Liver-specific transgenic expression of human NTCP in rhesus macaques confers HBV susceptibility on primary hepatocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CRISPR/Cas9 genome editing to create nonhuman primate models for studying stem cell therapies for HIV infection.Retrovirology · 2022Review
- Modeling genetic diseases in nonhuman primates through embryonic and germline modification: Considerations and challenges.Science translational medicine · 2022Review
- Gene editing monkeys: Retrospect and outlook.Frontiers in cell and developmental biology · 2022Review
- A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation.Scientific reports · 2021Article
- Comparative computer-assisted sperm analysis in non-human primates.Journal of medical primatology · 2021Article
- Alternative Animal Models of Aging Research.Frontiers in molecular biosciences · 2021Review
- Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset.ILAR journal · 2020Review
- Cryopreservation of Mauritian Cynomolgus Macaque (Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Local transgene expression and whole-body transgenesis to model brain diseases in nonhuman primate.Animal models and experimental medicine · 2019Review
- Deficiency of PRKD2 triggers hyperinsulinemia and metabolic disorders.Nature communications · 2018Article
- Trophoblast differentiation, invasion and hormone secretion in a three-dimensional in vitro implantation model with rhesus monkey embryos.Reproductive biology and endocrinology : RB&E · 2018Article
- Genetic engineering in nonhuman primates for human disease modeling.Journal of human genetics · 2018Review
- Assisted reproductive technologies in the common marmoset: an integral species for developing nonhuman primate models of human diseases.Biology of reproduction · 2017Review
- Brains, genes, and primates.Neuron · 2015Review
- TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys.Cell stem cell · 2014Article
- Highly efficient generation of transgenic sheep by lentivirus accompanying the alteration of methylation status.PloS one · 2013Article
- Review: Genetic manipulation of the rodent placenta.Placenta · 2011Review
- Transgenic rabbit production with simian immunodeficiency virus-derived lentiviral vector.Transgenic research · 2010Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Transgenic mice have provided invaluable information about gene function and regulation. However, because of marked differences between rodents and primates, some areas of human biology such as early embryonic development, aging, and maternal-fetal interactions would be best studied in a nonhuman primate model. Here, we report that gene transfer into rhesus monkey (Macaca mulatta) preimplantation embryos gives rise to transgenic placentas that express a reporter transgene (eGFP). Blastocysts resulting from culture of in vitro fertilized ova were transduced with a self-inactivating lentiviral vector and transferred into recipient females. One twin and one singleton pregnancy were produced from a single stimulation cycle, and one live rhesus monkey was born from each pregnancy. Placentas from all conceptuses showed expression of the transgene as detected by reverse transcription-PCR, ribonuclease protection assay, direct epifluorescence, immunohistochemistry, and Western blot analysis. Integration in somatic tissues of the offspring was not detected. A maternal immune response to the xenogeneic placental antigen was shown by the presence of anti-GFP antibodies in peripheral blood of the recipient females by day 99 of gestation (term = 165 days). These results demonstrate that transgene expression during gestation is compatible with successful pregnancy in nonhuman primates and provides an approach that could be broadly applicable to the development of novel models for primate biomedical research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.