ArticleProceedings of the National Academy of Sciences of the United States of America2001
Comprehensive survey of proteins targeted by chloroplast thioredoxin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers.
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Who cites it
135 citing papers in PubMed, 381 citations in OpenAlex.
- Allelic variation in the thioredoxin TRX-M4 influences carbon partitioning to control soluble sugar content in apple fruit.Plant communications · 2026Article
- Emerging roles of glutaredoxins in plant metabolism.Journal of experimental botany · 2026Review
- The Wheat NucleoredoxinPlants (Basel, Switzerland) · 2026Article
- Plastidial phosphoglucose isomerase undergoes thioredoxin-mediated redox modification without altering catalytic activity.The Biochemical journal · 2025Article
- Genetically encoding ε-N-methacryllysine into proteins in live cells.Nature communications · 2025Article
- Changes in the nitrate assimilation pathway serve as a component of maize stress response to competition cues.Frontiers in plant science · 2025Article
- Catalytic Activity of the Archetype from Group 4 of the FTR-like Ferredoxin:Thioredoxin Reductase Family Is Regulated by UniqueBiochemistry · 2024Article
- Current Insights into the Redox Regulation Network in Plant Chloroplasts.Plant & cell physiology · 2023Review
- Molecular mechanism of Rubisco activase: Dynamic assembly and Rubisco remodeling.Frontiers in molecular biosciences · 2023Review
- NewAntioxidants (Basel, Switzerland) · 2022Article
- Comprehensive Expression Analyses of Plastidial Thioredoxins ofAntioxidants (Basel, Switzerland) · 2022Article
- Cyclophilins and Their Functions in Abiotic Stress and Plant-Microbe Interactions.Biomolecules · 2021Review
- Repressed OsMESL expression triggers reactive oxygen species-mediated broad-spectrum disease resistance in rice.Plant biotechnology journal · 2021Article
- Functional redox links between lumen thiol oxidoreductase1 and serine/threonine-protein kinase STN7.Plant physiology · 2021Article
- Selective redox signaling shapes plant-pathogen interactions.Plant physiology · 2021Review
- 12-Frontiers in plant science · 2021Review
- Evaluation of CBSX Proteins as Regulators of the Chloroplast Thioredoxin System.Frontiers in plant science · 2021Article
- Article
- Collaboration between NDH and KEA3 Allows Maximally Efficient Photosynthesis after a Long Dark Adaptation.Plant physiology · 2020Article
- Biochemical insight into redox regulation of plastidial 3-phosphoglycerate dehydrogenase fromThe Journal of biological chemistry · 2020Article
75 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Possible target proteins of chloroplast thioredoxin (Trx) have been investigated in the stroma lysate of spinach chloroplasts. For that purpose, we immobilized a mutant of m-type Trx in which an internal cysteine at the active site was substituted with serine, on cyanogen bromide-activated resin. By using this resin, the target proteins in chloroplast were efficiently acquired when they formed the mixed-disulfide intermediates with the immobilized Trxs. We could acquire Rubisco activase (45 kDa) and 2-Cys-type peroxiredoxin (Prx), which were recently identified as targets of chloroplast Trxs. Glyceraldehyde-3-phosphate dehydrogenase and sedoheputulose 1,7-bisphosphatase, well-known thiol enzymes in the Calvin cycle, also were recognized among the collected proteins, suggesting the method is applicable for our purpose. Furthermore, four proteins were identified from a homology search of the NH(2)-terminal sequence of the acquired proteins: glutamine synthetase, a protein homologous to chloroplast cyclophilin, a homolog of Prx-Q, and the Rubisco small subunit. The Trx susceptibilities of the recombinant cyclophilin and Prx-Q of Arabidopsis thaliana were then examined. The method developed in the present study is thus applicable to investigate the various redox networks via Trxs and the related enzymes in the cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.