ArticleMolecular biology of the cell2001
Mediation of elicitin activity on tobacco is assumed by elicitin-sterol complexes.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Refractory Behavior in Plant Cells-Calcium Signaling Induced by Biotic Stress.Plants (Basel, Switzerland) · 2026Review
- Metabolomic Analysis of Phytophthora parasitica Growth in the Presence of β-sitosterol Indicates Adaptive Mechanisms Modulated by Sterols.Journal of basic microbiology · 2026Article
- Canopy insect communities are shaped by the genes and phenotypes of their aspen hosts.PloS one · 2025Article
- Genomic and transcriptomic analyses ofFrontiers in microbiology · 2024Article
- The function of plant PR1 and other members of the CAP protein superfamily in plant-pathogen interactions.Molecular plant pathology · 2023Review
- Unraveling Plant Cell Death duringMicroorganisms · 2022Review
- Bioprospecting of Rhizosphere-Resident Fungi: Their Role and Importance in Sustainable Agriculture.Journal of fungi (Basel, Switzerland) · 2021Review
- Organize, Don't Agonize: Strategic Success ofMicroorganisms · 2020Review
- Article
- Probing the Phylogenomics and Putative Pathogenicity Genes of Pythium insidiosum by Oomycete Genome Analyses.Scientific reports · 2018Article
- Phytophthora cinnamomi.Molecular plant pathology · 2018Article
- Quantitative Proteomic Analysis of Four Developmental Stages ofFrontiers in microbiology · 2017Article
- Plasma membrane order and fluidity are diversely triggered by elicitors of plant defence.Journal of experimental botany · 2016Article
- Elicitin-Induced Distal Systemic Resistance in Plants is Mediated Through the Protein-Protein Interactions Influenced by Selected Lysine Residues.Frontiers in plant science · 2016Article
- The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete Pythium insidiosum and evades host antibody responses.PloS one · 2015Article
- Ergosterol, an orphan fungal microbe-associated molecular pattern (MAMP).Molecular plant pathology · 2014Review
- Plant innate immunity: an updated insight into defense mechanism.Journal of biosciences · 2013Review
- Effect of plant sterols and tannins on Phytophthora ramorum growth and sporulation.Journal of chemical ecology · 2013Article
- Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance.Journal of experimental botany · 2012Article
- Sterol biosynthesis in oomycete pathogens.Plant signaling & behavior · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elicitins secreted by phytopathogenic Phytophthora spp. are proteinaceous elicitors of plant defense mechanisms and were demonstrated to load, carry, and transfer sterols between membranes. The link between elicitor and sterol-loading properties was assessed with the use of site-directed mutagenesis of the 47 and 87 cryptogein tyrosine residues, postulated to be involved in sterol binding. Mutated cryptogeins were tested for their ability to load sterols, bind to plasma membrane putative receptors, and trigger biological responses. For each mutated elicitin, the chemical characterization of the corresponding complexes with stigmasterol (1:1 stoichiometry) demonstrated their full functionality. However, these proteins were strongly altered in their sterol-loading efficiency, specific binding to high-affinity sites, and activities on tobacco cells. Ligand replacement experiments strongly suggest that the formation of a sterol-elicitin complex is a requisite step before elicitins fasten to specific binding sites. This was confirmed with the use of two sterol-preloaded elicitins. Both more rapidly displaced labeled cryptogein from its specific binding sites than the unloaded proteins. Moreover, the binding kinetics of elicitins are related to their biological effects, which constitutes the first evidence that binding sites could be the biological receptors. The first event involved in elicitin-mediated cell responses is proposed to be the protein loading with a sterol molecule.
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