ArticleMolecular biology of the cell2001
Sequential activities of phosphoinositide 3-kinase, PKB/Aakt, and Rab7 during macropinosome formation in Dictyostelium.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 76 citations in OpenAlex.
- Cross-Species Applications of Peptide Substrate Reporters to Quantitative Measurements of Kinase Activity.ACS measurement science au · 2024Article
- Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1.Molecular biology of the cell · 2022Article
- The PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium.Nature communications · 2022Article
- The Amoebal Model for Macropinocytosis.Sub-cellular biochemistry · 2022Article
- From Pinocytosis to Methuosis-Fluid Consumption as a Risk Factor for Cell Death.Frontiers in cell and developmental biology · 2021Review
- The Dual Role of Macropinocytosis in Cancers: Promoting Growth and Inducing Methuosis to Participate in Anticancer Therapies as Targets.Frontiers in oncology · 2020Review
- Article
- The origins and evolution of macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Akt and SGK protein kinases are required for efficient feeding by macropinocytosis.Journal of cell science · 2019Article
- GQDs-MSNs nanocomposite nanoparticles for simultaneous intracellular drug delivery and fluorescent imaging.Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology · 2018Article
- Akt3 kinase suppresses pinocytosis of low-density lipoprotein by macrophages via a novel WNK/SGK1/Cdc42 protein pathway.The Journal of biological chemistry · 2017Article
- CXCL12-induced macropinocytosis modulates two distinct pathways to activate mTORC1 in macrophages.Journal of leukocyte biology · 2017Article
- Productive Entry of Foot-and-Mouth Disease Virus via Macropinocytosis Independent of Phosphatidylinositol 3-Kinase.Scientific reports · 2016Article
- Differential signaling during macropinocytosis in response to M-CSF and PMA in macrophages.Frontiers in physiology · 2015Article
- A combination of SILAC and nucleotide acyl phosphate labelling reveals unexpected targets of the Rsk inhibitor BI-D1870.Bioscience reports · 2014Article
- Small GTPases and phosphoinositides in the regulatory mechanisms of macropinosome formation and maturation.Frontiers in physiology · 2014Review
- P2Y4 receptor-mediated pinocytosis contributes to amyloid beta-induced self-uptake by microglia.Molecular and cellular biology · 2013Article
- Two distinct functions for PI3-kinases in macropinocytosis.Journal of cell science · 2013Article
- Host cell entry of respiratory syncytial virus involves macropinocytosis followed by proteolytic activation of the F protein.PLoS pathogens · 2013Article
- Rabs and EHDs: alternate modes for traffic control.Bioscience reports · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Macropinocytosis plays an important role in the internalization of antigens by dendritic cells and is the route of entry for many bacterial pathogens; however, little is known about the molecular mechanisms that regulate the formation or maturation of macropinosomes. Like dendritic cells, Dictyostelium amoebae are active in macropinocytosis, and various proteins have been identified that contribute to this process. As described here, microscopic analysis of null mutants have revealed that the class I phosphoinositide 3-kinases, PIK1 and PIK2, and the downstream effector protein kinase B (PKB/Akt) are important in regulating completion of macropinocytosis. Although actin-rich membrane protrusions form in these cell lines, they recede without forming macropinosomes. Imaging of cells expressing green fluorescent protein (GFP) fused to the pleckstrin homology domain (PH) of PKB (GFP-PHPKB) indicates that D3 phosphoinositides are enriched in the forming macropinocytic cup and remain associated with newly formed macropinosomes for <1 minute. A fusion protein, consisting of GFP fused to an F-actin binding domain, overlaps with GFP-PHPKB in the timing of association with forming macropinosomes. Although macropinocytosis is reduced in cells expressing dominant negative Rab7, microscopic imaging studies reveal that GFP-Rab7 associates only with formed macropinosomes at approximately the time that F-actin and D3 phosphoinositide levels decrease. These results support a model in which F-actin modulating proteins and vesicle trafficking proteins coordinately regulate the formation and maturation of macropinosomes.
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