ArticleMolecular biology of the cell2001
Clathrin hub expression affects early endosome distribution with minimal impact on receptor sorting and recycling.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Targeting clathrin-mediated endocytosis: recent advances in inhibitor development, mechanistic insights, and therapeutic prospects.RSC medicinal chemistry · 2025Review
- DNAJC13 localization to endosomes is opposed by its J domain and its disordered C-terminus.Molecular biology of the cell · 2025Article
- DNAJC13 localization to endosomes is opposed by its J domain and its disordered C-terminal tail.bioRxiv : the preprint server for biology · 2024Article
- Clathrin Is Important for Virulence Factors Delivery in the Necrotrophic FungusFrontiers in plant science · 2021Article
- Wbox2: A clathrin terminal domain-derived peptide inhibitor of clathrin-mediated endocytosis.The Journal of cell biology · 2020Article
- Podocyte Lysosome Dysfunction in Chronic Glomerular Diseases.International journal of molecular sciences · 2020Review
- Clathrin terminal domain-ligand interactions regulate sorting of mannose 6-phosphate receptors mediated by AP-1 and GGA adaptors.The Journal of biological chemistry · 2014Article
- Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG.The Journal of cell biology · 2012Article
- Getting active: protein sorting in endocytic recycling.Nature reviews. Molecular cell biology · 2012Review
- Article
- The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step.The Journal of cell biology · 2010Article
- The function of the intermediate compartment in pre-Golgi trafficking involves its stable connection with the centrosome.Molecular biology of the cell · 2009Article
- Article
- Cell entry of arginine-rich peptides is independent of endocytosis.The Journal of biological chemistry · 2009Article
- In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5.Molecular biology of the cell · 2004Article
- The annexin 2/S100A10 complex controls the distribution of transferrin receptor-containing recycling endosomes.Molecular biology of the cell · 2003Article
- Clathrin-coated pits with long, dynamin-wrapped necks upon expression of a clathrin antisense RNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles.Molecular biology of the cell · 2002Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Clathrin-coated vesicles execute receptor-mediated endocytosis at the plasma membrane. However, a role for clathrin in later endocytic trafficking processes, such as receptor sorting and recycling or maintaining the organization of the endocytic pathway, has not been thoroughly characterized. The existence of clathrin-coated buds on endosomes suggests that clathrin might mediate later endocytic trafficking events. To investigate the function of clathrin-coated buds on endosomal membranes, endosome function and distribution were analyzed in a HeLa cell line that expresses the dominant-negative clathrin inhibitor Hub in an inducible manner. As expected, Hub expression reduced receptor-mediated endocytosis at the plasma membrane. Hub expression also induced a perinuclear aggregation of early endosome antigen 1-positive early endosomes, such that sorting and recycling endosomes were found tightly concentrated in the perinuclear region. Despite the dramatic redistribution of endosomes, Hub expression did not affect the overall kinetics of receptor sorting or recycling. These data show that clathrin function is necessary to maintain proper cellular distribution of early endosomes but does not play a prominent role in sorting and recycling events. Thus, clathrin's role on endosomal membranes is to influence organelle localization and is distinct from its role in trafficking pathways at the plasma membrane and trans-Golgi network.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.