ArticleMolecular biology of the cell2001
Myosin Va bound to phagosomes binds to F-actin and delays microtubule-dependent motility.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Many dynein teams collectively generate high forces during the transport of large organelles.Biophysical journal · 2026Article
- Ninein promotes F-actin cup formation and inward phagosome movement during phagocytosis in macrophages.Molecular biology of the cell · 2024Article
- Timing of Phagosome Maturation Depends on Their Transport Switching from Actin to Microtubule Tracks.The journal of physical chemistry. B · 2023Article
- Huntingtin S421 phosphorylation increases kinesin and dynein engagement on early endosomes and lysosomes.Biophysical journal · 2023Article
- Kinetics of phagosome maturation is coupled to their intracellular motility.Communications biology · 2022Article
- A guide to measuring phagosomal dynamics.The FEBS journal · 2021Review
- Better Together: Current Insights Into Phagosome-Lysosome Fusion.Frontiers in immunology · 2021Review
- Measuring Stepwise Binding of Thermally Fluctuating Particles to Cell Membranes without Fluorescence.Biophysical journal · 2020Article
- Squeezing in a Meal: Myosin Functions in Phagocytosis.Trends in cell biology · 2020Review
- Phagocytosis: Our Current Understanding of a Universal Biological Process.Frontiers in immunology · 2020Review
- Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.Molecular & cellular proteomics : MCP · 2019Article
- Phagosomal transport depends strongly on phagosome size.Scientific reports · 2017Article
- Novel antimitotic agents related to tubuloclustin: synthesis and biological evaluation.Molecular diversity · 2017Article
- Patterns, Receptors, and Signals: Regulation of Phagosome Maturation.Trends in immunology · 2017Review
- Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis.The Journal of cell biology · 2015Article
- Spatio-temporal model of endogenous ROS and raft-dependent WNT/beta-catenin signaling driving cell fate commitment in human neural progenitor cells.PLoS computational biology · 2015Article
- Reconstituting the motility of isolated intracellular cargoes.Methods in enzymology · 2014Article
- Force measurements on cargoes in living cells reveal collective dynamics of microtubule motors.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Autonomous phagosomal degradation and antigen presentation in dendritic cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A method for spatially resolved local intracellular mechanochemical sensing and organelle manipulation.Biophysical journal · 2012Article
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Authors and funding
11 authors.
Funding
Abstract
We established a light microscopy-based assay that reconstitutes the binding of phagosomes purified from mouse macrophages to preassembled F-actin in vitro. Both endogenous myosin Va from mouse macrophages and exogenous myosin Va from chicken brain stimulated the phagosome-F-actin interaction. Myosin Va association with phagosomes correlated with their ability to bind F-actin in an ATP-regulated manner and antibodies to myosin Va specifically blocked the ATP-sensitive phagosome binding to F-actin. The uptake and retrograde transport of phagosomes from the periphery to the center of cells in bone marrow macrophages was observed in both normal mice and mice homozygous for the dilute-lethal spontaneous mutation (myosin Va null). However, in dilute-lethal macrophages the accumulation of phagosomes in the perinuclear region occurred twofold faster than in normal macrophages. Motion analysis revealed saltatory phagosome movement with temporarily reversed direction in normal macrophages, whereas almost no reversals in direction were observed in dilute-lethal macrophages. These observations demonstrate that myosin Va mediates phagosome binding to F-actin, resulting in a delay in microtubule-dependent retrograde phagosome movement toward the cell center. We propose an "antagonistic/cooperative mechanism" to explain the saltatory phagosome movement toward the cell center in normal macrophages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.