ArticleInfection and immunity2001
Gonococcal genes encoding transferrin-binding proteins A and B are arranged in a bicistronic operon but are subject to differential expression.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Structural insights into the mechanism underpinning iron piracy in pathogenicScience advances · 2026Article
- Iron Reduction inInternational journal of molecular sciences · 2022Article
- Acclimation to Nutritional Immunity and Metal Intoxication Requires Zinc, Manganese, and Copper Homeostasis in the PathogenicFrontiers in cellular and infection microbiology · 2022Review
- Genome-wide discovery of epistatic loci affecting antibiotic resistance in Neisseria gonorrhoeae using evolutionary couplings.Nature microbiology · 2019Article
- Generation of Metal-Depleted Conditions for In Vitro Growth of Neisseria gonorrhoeae.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Subversion of nutritional immunity by the pathogenic Neisseriae.Pathogens and disease · 2018Review
- The genes that encode the gonococcal transferrin binding proteins, TbpB and TbpA, are differentially regulated by MisR under iron-replete and iron-depleted conditions.Molecular microbiology · 2016Article
- Reduction of the temperature sensitivity of Halomonas hydrothermalis by iron starvation combined with microaerobic conditions.Applied and environmental microbiology · 2015Article
- Identification of regulatory elements that control expression of the tbpBA operon in Neisseria gonorrhoeae.Journal of bacteriology · 2014Article
- Shared and distinct mechanisms of iron acquisition by bacterial and fungal pathogens of humans.Frontiers in cellular and infection microbiology · 2013Review
- The transferrin-iron import system from pathogenic Neisseria species.Molecular microbiology · 2012Review
- Evidence of Fe3+ interaction with the plug domain of the outer membrane transferrin receptor protein of Neisseria gonorrhoeae: implications for Fe transport.Metallomics : integrated biometal science · 2012Article
- TonB-Dependent Transporters Expressed by Neisseria gonorrhoeae.Frontiers in microbiology · 2011Article
- Metal uptake in host-pathogen interactions: role of iron in Porphyromonas gingivalis interactions with host organisms.Periodontology 2000 · 2010Review
- Transcriptional and functional analysis of the Neisseria gonorrhoeae Fur regulon.Journal of bacteriology · 2010Article
- Hijacking transferrin bound iron: protein-receptor interactions involved in iron transport in N. gonorrhoeae.Metallomics : integrated biometal science · 2009Article
- The lgtABCDE gene cluster, involved in lipooligosaccharide biosynthesis in Neisseria gonorrhoeae, contains multiple promoter sequences.Journal of bacteriology · 2004Article
- Transferrin-binding protein B of Neisseria meningitidis: sequence-based identification of the transferrin-Binding site confirmed by site-directed mutagenesis.Journal of bacteriology · 2004Article
- Growth of Neisseria gonorrhoeae in the female mouse genital tract does not require the gonococcal transferrin or hemoglobin receptors and may be enhanced by commensal lactobacilli.Infection and immunity · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Neisseria gonorrhoeae is capable of utilizing host iron-binding proteins, such as transferrin, lactoferrin, and hemoglobin, as the sole source of iron. The receptor involved in transferrin iron acquisition is composed of two distinct transferrin-binding proteins, TbpA and TbpB. The genes that encode these proteins are linked on the chromosome in the order tbpB-tbpA but are separated by an inverted repeat of unknown function. In this study, we sought to understand the transcriptional organization and regulation of the tbp genes, using a combination of lacZ transcriptional fusion analysis and reverse transcriptase PCR (RT-PCR). First, we demonstrated that tbpB and tbpA are cotranscribed and coregulated from the common upstream promoter that precedes tbpB. Using beta-galactosidase activity as a surrogate for tbp-specific transcription, we found that tbpB-specific transcripts were more prevalent than tbpA-specific transcripts after 2 h of growth under iron stress conditions. We confirmed the results obtained by fusion analysis by using RT-PCR applied to native RNA isolated from wild-type gonococci. Three different varieties of RT-PCR were employed: relative, competitive, and real time quantitative. The results of all analyses indicated that tbpB-specific transcripts were approximately twofold more prevalent than tbpA-specific transcripts at steady state. In iron-stressed cultures, the ratio of tbpB- to tbpA-specific message was approximately 2; however, in iron-replete cultures, this ratio dropped to 1. Using these techniques, we also quantitated the effects of iron, external pH, and presence of ligand on tbp mRNA levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.