ArticleInfection and immunity2001
Ferrochelatase is present in Brucella abortus and is critical for its intracellular survival and virulence.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
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- Colocalization and potential interactions ofScience advances · 2023Article
- Antibiogram Screening and Detection of Virulence-Associated Genes inInternational journal of environmental research and public health · 2022Article
- A comprehensive review of small regulatory RNAs inFrontiers in veterinary science · 2022Review
- Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis.Journal of fungi (Basel, Switzerland) · 2021Article
- Development of new generation of vaccines forHeliyon · 2018Review
- Development and trial of vaccines againstJournal of veterinary science · 2017Review
- [Drug resistance and protoporphyrin ferrochelatase ofZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2017Article
- Review
- Horizontally Acquired Biosynthesis Genes BoostFrontiers in cellular and infection microbiology · 2017Article
- Transition Metals and Virulence in Bacteria.Annual review of genetics · 2016Review
- Heme Synthesis and Acquisition in Bacterial Pathogens.Journal of molecular biology · 2016Review
- Global gene expression changes in Candidatus Liberibacter asiaticus during the transmission in distinct hosts between plant and insect.Molecular plant pathology · 2013Article
- Progress inFrontiers in biology · 2013Article
- Iron homeostasis in Brucella abortus: the role of bacterioferritin.Journal of microbiology (Seoul, Korea) · 2010Article
- Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi.Microbiology (Reading, England) · 2010Article
- Identifying essential genes in bacterial metabolic networks with machine learning methods.BMC systems biology · 2010Article
- Survival of the fittest: how Brucella strains adapt to their intracellular niche in the host.Medical microbiology and immunology · 2009Review
- Brucella abortus requires the heme transporter BhuA for maintenance of chronic infection in BALB/c mice.Infection and immunity · 2007Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella spp. are pathogenic bacteria that cause brucellosis, an animal disease which can also affect humans. Although understanding the pathogenesis is important for the health of animals and humans, little is known about virulence factors associated with it. In order for chronic disease to be established, Brucella spp. have developed the ability to survive inside phagocytes by evading cell defenses. It hides inside vacuoles, where it then replicates, indicating that it has an active metabolism. The purpose of this work was to obtain better insight into the intracellular metabolism of Brucella abortus. During a B. abortus genomic sequencing project, a clone coding a putative gene homologous to hemH was identified and sequenced. The amino acid sequence revealed high homology to members of the ferrochelatase family. A knockout mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALB/c mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B. abortus synthesizes its own heme and also has the ability to use an external source of heme; however, inside cells, there is not enough available heme to support its intracellular metabolism. It is concluded that ferrochelatase is essential for the multiplication and intracellular survival of B. abortus and thus for the establishment of chronic disease as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.