ArticleInfection and immunity2001
Identification of a new iron-regulated virulence gene, ireA, in an extraintestinal pathogenic isolate of Escherichia coli.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 128 citations in OpenAlex.
- The dual implications of iron homeostasis as a bacterial threat: its roles in virulence and antimicrobial resistance.Archives of microbiology · 2025Review
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- Impact of COVID-19 pandemic on antimicrobial resistance of Proteus mirabilis in a Brazilian hospital.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
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- Genomic diversity, pathogenicity and antimicrobial resistance of Escherichia coli isolated from poultry in the southern United States.BMC microbiology · 2023Article
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- Porcine extraintestinal pathogenicFrontiers in cellular and infection microbiology · 2023Article
- A Biomimetic Porcine Urothelial Model for AssessingMicroorganisms · 2022Article
- Antimicrobial Susceptibility and Detection of Virulence-Associated Genes inAntibiotics (Basel, Switzerland) · 2021Article
- Simultaneous Immunization with Multiple Diverse Immunogens Alters Development of Antigen-Specific Antibody-Mediated Immunity.Vaccines · 2021Article
- Enterobactin- and salmochelin-β-lactam conjugates induce cell morphologies consistent with inhibition of penicillin-binding proteins in uropathogenicChemical science · 2021Article
- Phylogenetic group determination and plasmid virulence gene profiles of colistin-resistantVeterinary world · 2020Article
- Optimization of an Experimental Vaccine To Prevent Escherichia coli Urinary Tract Infection.mBio · 2020Article
- Identification of ireA, 0007, 0008, and 2235 as TonB-dependent receptors in the avian pathogenic Escherichia coli strain DE205B.Veterinary research · 2020Article
- Pathogenicity Factors of Genomic Islands in Intestinal and ExtraintestinalFrontiers in microbiology · 2020Review
- Rapid Growth and Metabolism of Uropathogenic Escherichia coli in Relation to Urine Composition.Clinical microbiology reviews · 2019Review
- The iron hand of uropathogenic Escherichia coli: the role of transition metal control in virulence.Future microbiology · 2018Review
- Molecular Epidemiology of Extraintestinal PathogenicEcoSal Plus · 2018Review
- Comparison of Commensal Escherichia coli Isolates from Adults and Young Children in Lubuskie Province, Poland: Virulence Potential, Phylogeny and Antimicrobial Resistance.International journal of environmental research and public health · 2018Article
- Transcriptional Control of Dual Transporters Involved in α-Ketoglutarate Utilization Reveals Their Distinct Roles in UropathogenicFrontiers in microbiology · 2017Article
2 more citing papers are in PubMed but not listed here.
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3 authors at 2 institutions in 1 country.
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Abstract
Our laboratory is studying an extraintestinal pathogenic isolate of Escherichia coli (CP9) as a model pathogen. We have been using human urine, ascites, and blood ex vivo to identify genes with increased expression in these media relative to expression in Luria-Bertani (LB) broth. Such genes may represent new or unrecognized virulence traits. In this study, we report the identification of a new gene, ireA (iron-responsive element). This gene has an open reading frame of 2,049 nucleotides, and its peptide has a molecular mass of 75.3 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its expression is increased a mean of 3.6-fold in human urine, 16.2-fold in human ascites, and 6.6-fold in human blood relative to expression in LB medium, and it is Fe repressible. IreA also exhibits peptide similarities (48 to 56%) to previously identified proteins that function as siderophore receptors, suggesting that IreA is involved in iron acquisition. PCR-based analysis of ireA's phylogenetic distribution detected ireA in none (0%) of 14 fecal isolates that represented probable commensal strains, but in 13 (26%) of 50 random urine and blood clinical isolates (P = 0.05) and in 5 (100%) of 5 representatives of the J96-like, clonal group of which CP9 is a member (P < 0.001). In a mouse urinary tract infection model, the presence of ireA contributed significantly to CP9's ability to colonize the bladder (P < 0.02), evidence that IreA is a urovirulence factor. Taken together, these findings demonstrate that ireA encodes a new virulence factor, which is likely involved in Fe acquisition.
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