ArticleThe Plant cell2001
A 90-kD phospholipase D from tobacco binds to microtubules and the plasma membrane.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 258 citations in OpenAlex.
- Phosphatidic acid signaling in modulating plant reproduction and architecture.Plant communications · 2025Review
- Abscisic Acid, Microtubules and Phospholipase D-Solving a Cellular Bermuda Triangle.International journal of molecular sciences · 2024Article
- Exploration of the pearl millet phospholipase gene family to identify potential candidates for grain quality traits.BMC genomics · 2024Article
- Phospholipase Dδ and phosphatidic acid mediate heat-induced nuclear localization of glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis.The Plant journal : for cell and molecular biology · 2022Article
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- Plant cell polarity as the nexus of tissue mechanics and morphogenesis.Nature plants · 2021Review
- The Reduced Longitudinal Growth Induced by Overexpression ofPlants (Basel, Switzerland) · 2021Article
- HSP70-3 Interacts with Phospholipase Dδ and Participates in Heat Stress Defense.Plant physiology · 2021Article
- Link between Lipid Second Messengers and Osmotic Stress in Plants.International journal of molecular sciences · 2021Review
- Exogenous Auxin Induces Transverse Microtubule Arrays Through TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX Receptors.Plant physiology · 2020Article
- Secretion of Phospholipase Dδ Functions as a Regulatory Mechanism in Plant Innate Immunity.The Plant cell · 2019Article
- A DREPP protein interacted with PeaT1 from Alternaria tenuissima and is involved in elicitor-induced disease resistance in Nicotiana plants.Journal of plant research · 2018Article
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- The self-organization of plant microtubules inside the cell volume yields their cortical localization, stable alignment, and sensitivity to external cues.PLoS computational biology · 2018Article
- Update: Plant Cortical Microtubule Arrays.Plant physiology · 2018Review
- Emerging roles of cortical microtubule-membrane interactions.Journal of plant research · 2018Review
- Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy.Frontiers in plant science · 2018Article
- An Overview of Signaling Regulons During Cold Stress Tolerance in Plants.Current genomics · 2017Review
- Lipid remodelling: Unravelling the response to cold stress in Arabidopsis and its extremophile relative Eutrema salsugineum.Plant science : an international journal of experimental plant biology · 2017Review
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The organization of microtubule arrays in the plant cell cortex involves interactions with the plasma membrane, presumably through protein bridges. We have used immunochemistry and monoclonal antibody 6G5 against a candidate bridge protein, a 90-kD tubulin binding protein (p90) from tobacco BY-2 membranes, to characterize the protein and isolate the corresponding gene. Screening an Arabidopsis cDNA expression library with the antibody 6G5 produced a partial clone encoding phospholipase D (PLD), and a full-length gene was obtained by sequencing a corresponding expressed sequence tag clone. The predicted protein of 857 amino acids contains the active sites of a phospholipid-metabolizing enzyme and a Ca(2+)-dependent lipid binding domain and is identical to Arabidopsis PLD delta. Two amino acid sequences obtained by Edman degradation of the tobacco p90 are identical to corresponding segments of a PLD sequence from tobacco. Moreover, immunoprecipitation using the antibody 6G5 and tobacco BY-2 protein extracts gave significant PLD activity, and PLD activity of tobacco BY-2 membrane proteins was enriched 6.7-fold by tubulin-affinity chromatography. In a cosedimentation assay, p90 bound and decorated microtubules. In immunofluorescence microscopy of intact tobacco BY-2 cells or lysed protoplasts, p90 colocalized with cortical microtubules, and taxol-induced microtubule bundling was accompanied by corresponding reorganization of p90. Labeling of p90 remained along the plasma membrane when microtubules were depolymerized, although detergent extraction abolished the labeling. Therefore, p90 is a specialized PLD that associates with membranes and microtubules, possibly conveying hormonal and environmental signals to the microtubule cytoskeleton.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.