ArticleBMC physiology2001
1,25-Dihydroxyvitamin D3 increases the expression of the CaT1 epithelial calcium channel in the Caco-2 human intestinal cell line.
Article in BMC physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 850 citations in OpenAlex.
- The effect of vitamin D2 and vitamin D3 on intestinal calcium absorption in Nigerian children with rickets.The Journal of clinical endocrinology and metabolism · 2009Trial
- Calcium selective channel TRPV6: Structure, function, and implications in health and disease.Gene · 2022Review
- Analysis of 1,25-Dihydroxyvitamin DMolecular and cellular biology · 2020Article
- Myosin 1a Regulates Osteoblast Differentiation Independent of Intestinal Calcium Transport.Journal of the Endocrine Society · 2019Article
- Development of a Whole Organism Platform for Phenotype-Based Analysis of IGF1R-PI3K-Akt-Tor Action.Scientific reports · 2017Article
- Effect of additive calcium administration on FGF23 levels in patients with mild chronic kidney disease treated with calcitriol: a randomized, open-labeled clinical trial.Therapeutics and clinical risk management · 2017Article
- Vitamin D: recent advances and implications for athletes.Sports medicine (Auckland, N.Z.) · 2015Review
- Calcium deficiency-induced and TRP channel-regulated IGF1R-PI3K-Akt signaling regulates abnormal epithelial cell proliferation.Cell death and differentiation · 2014Article
- Influence of 1α, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes.Journal of animal science and technology · 2014Article
- Comparison between perioperative treatment with calcium and with calcium and vitamin d in prevention of post thyroidectomy hypocalcemia.Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India · 2014Article
- A Nonsecosteroidal Vitamin D Receptor Modulator Ameliorates Experimental Autoimmune Encephalomyelitis without Causing Hypercalcemia.Autoimmune diseases · 2011Article
- Down-regulation of intestinal apical calcium entry channel TRPV6 by ubiquitin E3 ligase Nedd4-2.The Journal of biological chemistry · 2010Article
- Expression of epithelial calcium transport system in rat cochlea and vestibular labyrinth.BMC physiology · 2010Article
- Human duodenum responses to vitamin D metabolites of TRPV6 and other genes involved in calcium absorption.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- WNK3 positively regulates epithelial calcium channels TRPV5 and TRPV6 via a kinase-dependent pathway.American journal of physiology. Renal physiology · 2008Article
- Interaction of the epithelial Ca2+ channels TRPV5 and TRPV6 with the intestine- and kidney-enriched PDZ protein NHERF4.Pflugers Archiv : European journal of physiology · 2006Article
- Effect of 17beta-oestradiol on transepithelial calcium transport in human intestinal-like Caco-2 cells and its interactions with 1,25-dihydroxycholecalciferol and 9-cis retinoic acid.European journal of nutrition · 2006Article
- 1,25-Dihydroxyvitamin D and 25-hydroxyvitamin D--mediated regulation of TRPV6 (a putative epithelial calcium channel) mRNA expression in Caco-2 cells.European journal of nutrition · 2006Article
- Calcium transport in human salivary glands: a proposed model of calcium secretion into saliva.Histochemistry and cell biology · 2006Article
- Identification and characterization of noncalcemic, tissue-selective, nonsecosteroidal vitamin D receptor modulators.The Journal of clinical investigation · 2006Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe active hormonal form of vitamin D (1,25-dihydroxyvitamin D) is the primary regulator of intestinal calcium absorption efficiency. In vitamin D deficiency, intestinal calcium absorption is low leading to an increased risk of developing negative calcium balance and bone loss. 1,25-dihydroxyvitamin D has been shown to stimulate calcium absorption in experimental animals and in human subjects. However, the molecular details of calcium transport across the enterocyte are not fully defined. Recently, two novel epithelial calcium channels (CaT1/ECaC2 and ECaC1/CaT2) have been cloned and suggested to be important in regulating intestinal calcium absorption. However, to date neither gene has been shown to be regulated by vitamin D status. We have previously shown that 1,25-dihydroxyvitamin stimulates transcellular calcium transport in Caco-2 cells, a human intestinal cell line.
resultsIn the current study, we have demonstrated that Caco-2 cells express low but detectable levels of CaT1 mRNA in the absence of 1,25-dihydroxyvitamin D treatment. CaT1 mRNA expression is rapidly up regulated (4-fold increase at 4 h and 10-fold at 24 h) by treatment with 1,25-dihydroxyvitamin D (10(-7) moles/L). Moreover, the increase in CaT1 mRNA expression preceded by several hours the vitamin D induction of calbindin D9K, a putative cytosolic calcium transport protein.
conclusionThese observations are the first to demonstrate regulation of CaT1 expression by vitamin D and are consistent with a new model of intestinal calcium absorption wherein vitamin D-mediated changes in brush border membrane CaT1 levels could be the primary gatekeeper regulating homeostatic modulation of intestinal calcium absorption efficiency.
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