ArticleBMC microbiology2001
MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis.
Article in BMC microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 30 citations in OpenAlex.
- Article
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- A bifunctional salvage pathway for two distinct S-adenosylmethionine by-products that is widespread in bacteria, including pathogenic Escherichia coli.Molecular microbiology · 2020Article
- Revisiting the methionine salvage pathway and its paralogues.Microbial biotechnology · 2019Article
- Article
- MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila.PloS one · 2013Article
- A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis.Nature chemical biology · 2012Article
- Identification of Rv0535 as methylthioadenosine phosphorylase from Mycobacterium tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2012Article
- Structure of the antibiotic resistance factor spectinomycin phosphotransferase from Legionella pneumophila.The Journal of biological chemistry · 2010Article
- Structure of Arabidopsis thaliana 5-methylthioribose kinase reveals a more occluded active site than its bacterial homolog.BMC structural biology · 2007Article
- Structural and functional diversity of the microbial kinome.PLoS biology · 2007Article
- Identification of a protein subset of the anthrax spore immunome in humans immunized with the anthrax vaccine adsorbed preparation.Infection and immunity · 2005Article
- Functional analysis of methylthioribose kinase genes in plants.Plant physiology · 2004Article
- Bacterial variations on the methionine salvage pathway.BMC microbiology · 2004Article
- Methionine regeneration and aminotransferases in Bacillus subtilis, Bacillus cereus, and Bacillus anthracis.Journal of bacteriology · 2003Article
- Global expression profile of Bacillus subtilis grown in the presence of sulfate or methionine.Journal of bacteriology · 2002Article
- The methionine salvage pathway in Bacillus subtilis.BMC microbiology · 2002Article
- Prediction of gene function in methylthioadenosine recycling from regulatory signals.Journal of bacteriology · 2002Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMethylthioadenosine, the main by-product of spermidine synthesis, is degraded in Bacillus subtilis as adenine and methylthioribose. The latter is an excellent sulfur source and the precursor of quorum-sensing signalling molecules. Nothing was known about methylthioribose recycling in this organism.
resultsUsing trifluoromethylthioribose as a toxic analog to select for resistant mutants, we demonstrate that methylthioribose is first phosphorylated by MtnK, methylthioribose kinase, the product of gene mtnK (formerly ykrT), expressed as an operon with mtnS (formerly ykrS) in an abundant transcript with a S-box leader sequence. Although participating in methylthioribose recycling, the function of mtnS remained elusive. We also show that MtnK synthesis is boosted under starvation condition, in the following decreasing order: carbon-, sulfur- and nitrogen-starvation. We finally show that this enzyme is part of the family Pfam 01633 (choline kinases) which belongs to a large cluster of orthologs comprizing antibiotic aminoglycoside kinases and protein serine/threonine kinases.
conclusionsThe first step of methylthioribose recycling is phosphorylation by MTR kinase, coded by the mtnK (formerly ykrT) gene. Analysis of the neighbourhood of mtnK demonstrates that genes located in its immediate vicinity (now named mtnUVWXYZ, formerly ykrUVWXYZ) are also required for methylthioribose recycling.
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