ArticleJournal of bacteriology2001
Juxtaposition of an active promoter to vsp genes via site-specific DNA inversions generates antigenic variation in Mycoplasma bovis.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Transcriptomic analysis of naturally occurring bovine respiratory disease in Australian feedlot cattle identifies bovine viral diarrhoea virus-induced immunosuppression and Mollicutes as the dominant drivers of lung inflammation.Veterinary research · 2026Article
- The Phenotypic Divergence and Potential Microevolution of a DominantMicroorganisms · 2026Article
- De novo genome assembly resolving repetitive structures enables genomic analysis of 35 European Mycoplasmopsis bovis strains.BMC genomics · 2023Article
- Regularity of Toll-Like Receptors in Bovine Mammary Epithelial Cells Induced byFrontiers in veterinary science · 2022Article
- TheAntibiotics (Basel, Switzerland) · 2021Article
- Molecular Mechanisms ofJournal of bacteriology · 2019Article
- The Performance of Three Immune Assays to Assess the Serological Status of Cattle Experimentally Exposed to Mycoplasma bovis.Veterinary sciences · 2018Article
- Disruption of the membrane nuclease gene (MBOVPG45_0215) of Mycoplasma bovis greatly reduces cellular nuclease activity.Journal of bacteriology · 2015Article
- Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172.Molecular microbiology · 2011Article
- Complete genome sequence of Mycoplasma bovis type strain PG45 (ATCC 25523).Infection and immunity · 2011Article
- Common strategies for antigenic variation by bacterial, fungal and protozoan pathogens.Nature reviews. Microbiology · 2009Review
- Occurrence, plasticity, and evolution of the vpma gene family, a genetic system devoted to high-frequency surface variation in Mycoplasma agalactiae.Journal of bacteriology · 2009Article
- Multiple genome comparison within a bacterial species reveals a unit of evolution spanning two adjacent genes in a tandem paralog cluster.Molecular biology and evolution · 2008Article
- Genome of Mycoplasma arthritidis.Infection and immunity · 2008Article
- Phase-locked mutants of Mycoplasma agalactiae: defining the molecular switch of high-frequency Vpma antigenic variation.Molecular microbiology · 2008Article
- Array-based genomic comparative hybridization analysis of field strains of Mycoplasma hyopneumoniae.Journal of bacteriology · 2007Article
- Being pathogenic, plastic, and sexual while living with a nearly minimal bacterial genome.PLoS genetics · 2007Article
- Avoidance of the host immune system through phase variation in Mycoplasma pulmonis.Infection and immunity · 2005Article
- Molecular epidemiological analysis of Mycoplasma bovis isolates from the United Kingdom shows two genetically distinct clusters.Journal of clinical microbiology · 2004Article
- Phase and antigenic variation in bacteria.Clinical microbiology reviews · 2004Review
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3 authors.
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Abstract
Mycoplasma bovis, the most important etiological agent of bovine mycoplasmosis, undergoes extensive antigenic variation of major and highly immunogenic surface lipoprotein antigens (Vsps). A family of 13 related but divergent vsp genes, which occur as single chromosomal copies, was recently found in the chromosome of M. bovis. In the present study, the molecular mechanism mediating the high-frequency phase variation of two Vsps (VspA and VspC) as representatives of the Vsp family was investigated. Analysis of clonal isolates exhibiting phase transitions of VspA or of VspC (i.e., ON-->OFF-->ON) has shown that DNA inversions occur during Vsp phase variation. The upstream region of each vsp gene contains two sequence cassettes. The first (cassette no. 1), a 71-bp region upstream of the ATG initiation codon, exhibits 98% homology among all vsp genes, while the second (cassette no. 2), upstream of cassette no. 1, ranges in size from 50 to 180 bp and is more divergent. Examination of the ends of the inverted fragments during VspA or VspC phase variation revealed that in both cases, a change in the organization of vsp upstream cassettes involving three vsp genes had occurred. Primer extension and Northern blot analysis have shown that a specific cassette no. 2, designated A(2), is an active promoter and that juxtaposition of this regulatory element to a silent vsp gene by DNA inversions allows transcription initiation of the recipient gene. Further genetic analysis revealed that phase variation of VspA or of VspC involves two site-specific DNA inversions occurring between inverted copies of a specific 35-bp sequence present within the conserved cassette no. 1. A model for the control of Vsp phase variation is proposed.
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