ArticleJournal of bacteriology2001
Enhancer-binding proteins HrpR and HrpS interact to regulate hrp-encoded type III protein secretion in Pseudomonas syringae strains.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 158 citations in OpenAlex.
- Novel insights into the genetic basis and transcriptomic elucidation of virulence attenuation inVirulence · 2025Article
- VimR is a conserved master transcription regulator controlling virulence and metabolism in Pseudomonas.Nucleic acids research · 2025Article
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- GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogenFrontiers in plant science · 2024Article
- Type III Secretion System Repressor RhpR Induces GrlP, a Glycine-Rich Outer Membrane Lipoprotein with Functions in Regulating the Periplasmic Space and Pleiotropic Responses.Applied and environmental microbiology · 2023Article
- RpoN is required for the motility and contributes to the killing ability of Plesiomonas shigelloides.BMC microbiology · 2022Article
- The OmpR-like Transcription Factor as a Negative Regulator ofInternational journal of molecular sciences · 2022Article
- Hierarchical regulation of Burkholderia glumae type III secretion system by GluR response regulator and Lon protease.Molecular plant pathology · 2022Article
- Maintenance of tRNA and elongation factors supports T3SS proteins translational elongations in pathogenic bacteria during nutrient starvation.Cell & bioscience · 2022Article
- Interactions between paralogous bacterial enhancer-binding proteins enable metal-dependent regulation of alternative nitrogenases in Azotobacter vinelandii.Molecular microbiology · 2022Article
- Virulence-related regulatory network ofComputational and structural biotechnology journal · 2022Review
- Pseudomonas syringae senses polyphenols via phosphorelay crosstalk to inhibit virulence.EMBO reports · 2021Article
- The Regulatory Functions of σInternational journal of molecular sciences · 2021Review
- HrpL Regulon of Bacterial Pathogen of Woody HostMicroorganisms · 2021Article
- Regulation of theMicroorganisms · 2021Review
- Article
- Ancient co-option of an amino acid ABC transporter locus in Pseudomonas syringae for host signal-dependent virulence gene regulation.PLoS pathogens · 2020Article
- Signal transduction schemes inComputational and structural biotechnology journal · 2020Review
- Comparative genomics reveal pathogenicity-related loci in Pseudomonas syringae pv. actinidiae biovar 3.Molecular plant pathology · 2019Article
- Two components of the rhpPC operon coordinately regulate the type III secretion system and bacterial fitness in Pseudomonas savastanoi pv. phaseolicola.PLoS pathogens · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Pseudomonas syringae strains, the hrp-hrc pathogenicity island consists of an HrpL-dependent regulon that encodes a type III protein translocation complex and translocated effector proteins required for pathogenesis. HrpR and HrpS function as positive regulatory factors for the hrpL promoter, but their mechanism of action has not been established. Both HrpR and HrpS are structurally related to enhancer-binding proteins, but they lack receiver domains and do not appear to require a cognate protein kinase for activity. hrpR and hrpS were shown to be expressed as an operon: a promoter was identified 5' to hrpR, and reverse transcriptase PCR detected the presence of an hrpRS transcript. The hrpR promoter and coding sequence were conserved among P. syringae strains. The coding sequences for hrpR and hrpS were cloned into compatible expression vectors, and their activities were monitored in Escherichia coli transformants carrying an hrpL'-lacZ fusion. HrpS could function as a weak activator of the hrpL promoter, but the activity was only 2.5% of the activity detected when both HrpR and HrpS were expressed in the reporter strain. This finding is consistent with a requirement for both HrpR and HrpS in the activation of the hrpL promoter. By using a yeast two-hybrid assay, an interaction between HrpR and HrpS was detected, suggestive of the formation of a heteromeric complex. Physical interaction of HrpR and HrpS was confirmed by column-binding experiments. The results show that HrpR and HrpS physically interact to regulate the sigma(54)-dependent hrpL promoter in P. syringae strains.
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