ArticleJournal of bacteriology2001
Positive selection of mutations leading to loss or reduction of transcriptional activity of PrfA, the central regulator of Listeria monocytogenes virulence.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- The LhrC sRNAs control expression of T cell-stimulating antigen TcsA in Listeria monocytogenes by decreasing tcsA mRNA stability.RNA biology · 2019Article
- Spontaneous Loss of Virulence in Natural Populations of Listeria monocytogenes.Infection and immunity · 2017Article
- Genome comparison of Listeria monocytogenes serotype 4a strain HCC23 with selected lineage I and lineage II L. monocytogenes strains and other Listeria strains.Genomics data · 2014Article
- The structure of Bradyrhizobium japonicum transcription factor FixK2 unveils sites of DNA binding and oxidation.The Journal of biological chemistry · 2013Article
- A novel C-terminal mutation resulting in constitutive activation of the Listeria monocytogenes central virulence regulatory factor PrfA.Microbiology (Reading, England) · 2011Article
- The virulence regulator PrfA promotes biofilm formation by Listeria monocytogenes.Journal of bacteriology · 2010Article
- Point mutations within the streptococcal regulator of virulence (Srv) alter protein-DNA interactions and Srv function.Microbiology (Reading, England) · 2008Article
- Species-specific differences in the activity of PrfA, the key regulator of listerial virulence genes.Journal of bacteriology · 2006Article
- How the bacterial pathogen Listeria monocytogenes mediates the switch from environmental Dr. Jekyll to pathogenic Mr. Hyde.Infection and immunity · 2006Review
- Identification of srv, a PrfA-like regulator of group A streptococcus that influences virulence.Infection and immunity · 2004Article
- Multi-virulence-locus sequence typing of Listeria monocytogenes.Applied and environmental microbiology · 2004Article
- Deletion of the gene encoding p60 in Listeria monocytogenes leads to abnormal cell division and loss of actin-based motility.Infection and immunity · 2003Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factor PrfA controls the expression of virulence genes essential for Listeria monocytogenes pathogenesis. To gain insight into the structure-function relationship of PrfA, we devised a positive-selection system to isolate mutations reducing or abolishing transcriptional activity. The system is based on the observation that the listerial iap gene, encoding the p60 protein, is lethal if overexpressed in Bacillus subtilis. A plasmid in which the iap gene is placed under the control of the PrfA-dependent hly promoter was constructed and introduced into B. subtilis. This strain was rapidly killed when expression of iap was induced by introduction of a second plasmid carrying prfA. Two classes of B. subtilis survivor mutants were identified: one carried mutations in iap, and the second carried mutations in prfA. Sequence analysis of the defective prfA genes identified mutations in three regions of the PrfA protein: region A, between amino acids 58 and 67 in the beta-roll domain of PrfA; region B, between amino acids 169 and 193, which corresponds to the DNA-binding helix-turn-helix motif; and region C, comprising the 38 C-terminal amino acids of PrfA, which form a leucine zipper-like structure. PrfA proteins with mutations in regions B and C were unable to bind to the PrfA-binding site in the target DNA, while mutations in region A resulted in a protein still binding the target DNA but unable to form a stable complex with RNA polymerase and initiate transcription in vitro.
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