ArticleJournal of bacteriology2001
Autophosphorylation of a bacterial serine/threonine kinase, AfsK, is inhibited by KbpA, an AfsK-binding protein.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- The roles of SARP family regulators involved in secondary metabolism inFrontiers in microbiology · 2024Review
- Functional investigation of AfsKRS regulatory system for pristinamycin biosynthesis in3 Biotech · 2021Article
- Modulation of Gene Expression in Actinobacteria by Translational Modification of Transcriptional Factors and Secondary Metabolite Biosynthetic Enzymes.Frontiers in microbiology · 2021Review
- The Balance Metabolism Safety Net: Integration of Stress Signals by Interacting Transcriptional Factors inFrontiers in microbiology · 2019Review
- Molecular regulation of antibiotic biosynthesis in streptomyces.Microbiology and molecular biology reviews : MMBR · 2013Review
- A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence.BMC genomics · 2011Article
- Leaderless genes in bacteria: clue to the evolution of translation initiation mechanisms in prokaryotes.BMC genomics · 2011Article
- Complex transcriptional control of the antibiotic regulator afsS in Streptomyces: PhoP and AfsR are overlapping, competitive activators.Journal of bacteriology · 2011Article
- Eukaryote-like serine/threonine kinases and phosphatases in bacteria.Microbiology and molecular biology reviews : MMBR · 2011Review
- New Test System for Serine/Threonine Protein Kinase Inhibitors Screening: E. coli APHVIII/Pk25 design.Acta naturae · 2010Article
- Characterization of rrdA, a TetR family protein gene involved in the regulation of secondary metabolism in Streptomyces coelicolor.Applied and environmental microbiology · 2009Article
- Genome-wide transcriptome analysis reveals that a pleiotropic antibiotic regulator, AfsS, modulates nutritional stress response in Streptomyces coelicolor A3(2).BMC genomics · 2008Article
- Phosphorylated AbsA2 negatively regulates antibiotic production in Streptomyces coelicolor through interactions with pathway-specific regulatory gene promoters.Journal of bacteriology · 2007Article
- Catalytic domain of AfsKav modulates both secondary metabolism and morphologic differentiation in Streptomyces avermitilis ATCC 31272.Current microbiology · 2006Article
- Diversity in domain architectures of Ser/Thr kinases and their homologues in prokaryotes.BMC genomics · 2005Article
- AfsR as an integrator of signals that are sensed by multiple serine/threonine kinases in Streptomyces coelicolor A3(2).Journal of industrial microbiology & biotechnology · 2003Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A protein serine/threonine kinase, AfsK, and its target protein AfsR globally control physiological and morphological differentiation in the bacterial genus Streptomyces. A protein (KbpA) of 252 amino acids encoded by an open reading frame in a region upstream of afsK in Streptomyces coelicolor A3(2) was identified as an AfsK-interacting protein. The interaction site of AfsK was in the N-terminal portion containing the kinase catalytic domain. KbpA bound a nonphosphorylated form of AfsK and inhibited its autophosphorylation at serine and threonine residues. KbpA in the reaction mixture containing AfsK and AfsR also inhibited the phosphorylation of AfsR by AfsK, presumably because KbpA inhibited the conversion from the inactive, nonphosphorylated form of AfsK to the active, phosphorylated form. kbpA was transcribed throughout growth, and the transcription was enhanced when production of actinorhodin had already started. KbpA thus appeared to play an inhibitory role in a negative feedback system in the AfsK-AfsR regulatory pathway. Consistent with these in vitro observations, kbpA served as a repressor for actinorhodin production in S. coelicolor A3(2); disruption of kbpA greatly enhanced actinorhodin production, and overexpression of kbpA reduced the production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.