ArticlePlant physiology1996
Laser microsurgery of higher plant cell walls permits patch-clamp access.
Article in Plant physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi.Microsystems & nanoengineering · 2024Article
- Gadolinium effects on gigaseal formation and the adhesive properties of a fungal amoeboid cell, the slime mutant of Neurospora crassa.The Journal of membrane biology · 2004Article
- Dominant negative guard cell K+ channel mutants reduce inward-rectifying K+ currents and light-induced stomatal opening in arabidopsis.Plant physiology · 2001Article
- A laser microsurgical method of cell wall removal allows detection of large-conductance ion channels in the guard cell plasma membrane.Protoplasma · 1999Article
- Expression of a Cs(+)-resistant guard cell K+ channel confers Cs(+)-resistant, light-induced stomatal opening in transgenic arabidopsis.The Plant cell · 1997Article
- Roles of higher plant K+ channels.Plant physiology · 1997Review
- Patch-clamping and other molecular approaches for the study of plasma membrane transporters demystified.Plant physiology · 1997Review
- Photosynthetic Electron Transport in Single Guard Cells as Measured by Scanning Electrochemical Microscopy.Plant physiology · 1997Article
- Spatial Organization of Calcium Signaling Involved in Cell Volume Control in the Fucus Rhizoid.The Plant cell · 1996Article
- Laser selection significantly affects cell viability following single-cell nanosurgery.Photochemistry and photobiologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Plasma membranes of guard cells in epidermal peels of Vicia faba and Commelina communis can be made accessible to a patch-clamp pipet by removing a small portion (1-3 micrometers in diameter) of the guard cell wall using a microbeam of ultraviolet light generated by a nitrogen laser. Using this laser microsurgical technique, we have measured channel activity across plasma membranes of V. faba guard cells in both cell-attached and isolated patch configurations. Measurements made in the inside-out patch configuration revealed two distinct K(+)-selective channels. Major advantages of the laser microsurgical technique include the avoidance of enzymatic protoplast isolation, the ability to study cell types that have been difficult to isolate as protoplasts or for which enzymatic isolation protocols result in protoplasts not amenable to patch-clamp studies, the maintenance of positional information in single-channel measurements, reduced disruption of cell-wall-mediated signaling pathways, and the ability to investigate intercellular signaling through studies of cells remaining situated within tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.