Evidence map›Paper›PMID 11536654›Full record

ArticleThe Plant cell1994

NPA binding activity is peripheral to the plasma membrane and is associated with the cytoskeleton.

D N Cox, G K Muday

Open access · bronzeAbstract read
In one paragraph

Article in The Plant cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
3.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 75 citations in OpenAlex.

  1. Review
  2. A Retro-Perspective on Auxin Transport.Frontiers in plant science · 2021
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  6. Review
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  19. ARG1 (altered response to gravity) encodes a DnaJ-like protein that potentially interacts with the cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 1999
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

D N CoxWake Forest University, Department of Biology, Winston-Salem, North Carolina 27109, USA.
G K Muday
Wake Forest University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

N-1-Naphthylphthalamic acid (NPA) binding activity is released into the supernatant when plasma membranes are subjected to high-salt treatment, indicating that this activity is peripherally associated with the membrane. Extraction of plasma membrane vesicles with Triton X-100 resulted in retention of NPA binding activity in the detergent-insoluble cytoskeletal pellet. Treatment of this pellet with KI released NPA binding activity, actin, and alpha-tubulin. Dialysis to remove KI led to the repolymerization of cytoskeletal elements and movement of NPA binding activity into an insoluble cytoskeletal pellet. NPA binding activity partitioned into the detergent-insoluble cytoskeletal pellet obtained from both zucchini and maize membranes and was released from these pellets by KI treatment. Treatment of a cytoskeletal pellet with cytochalasin B doubled NPA binding activity in the resulting supernatant. Together, these experiments indicate that NPA binding activity is peripherally associated with the plasma membrane and interacts with the cytoskeleton in vitro.

Indexed as

ActinsBinding SitesBiological TransportCarbonatesCarrier ProteinsCell MembraneCucurbitaceaeCytochalasin BCytoskeletonDetergentsIndoleacetic AcidsOctoxynolPhthalimidesPlant ProteinsPotassium IodideProtein BindingActinsalpha-naphthylphthalamic acidCarbonatesCarrier ProteinsCytochalasin BDetergentsIndoleacetic AcidsOctoxynolPhthalimidesPlant ProteinsPotassium Iodidesodium carbonate

Identifiers

PMID11536654
PMCPMC160574
OpenAlexW2059457598

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.