ArticleProceedings of the National Academy of Sciences of the United States of America2001
Single-cell analysis of signal transduction in CD4 T cells stimulated by antigen in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
17 citing papers in PubMed, 76 citations in OpenAlex.
- Single-cell RNA sequencing reveals Immune Education promotes T cell survival in mice subjected to the cecal ligation and puncture sepsis model.Frontiers in immunology · 2024Article
- Intracellular Flow Cytometry ("Phosphoflow") to Assess Signal Transduction in Rare Populations Such As Memory B Cell Subsets and Plasma Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Superresolution imaging reveals nanometer- and micrometer-scale spatial distributions of T-cell receptors in lymph nodes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Multiparameter flow cytometry: advances in high resolution analysis.Immune network · 2013Article
- Early in vivo signaling profiles in MUC1-specific CD4Oncoimmunology · 2013Article
- Role of antigen persistence and dose for CD4+ T-cell exhaustion and recovery.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Development and application of 'phosphoflow' as a tool for immunomonitoring.Expert review of vaccines · 2010Review
- Ethanol inhibits LPS-induced signaling and modulates cytokine production in peritoneal macrophages in vivo in a model for binge drinking.BMC immunology · 2009Article
- STAT-3 and ERK 1/2 phosphorylation are critical for T-cell alloactivation and graft-versus-host disease.Blood · 2008Article
- Incorporation of membrane-anchored flagellin into influenza virus-like particles enhances the breadth of immune responses.Journal of virology · 2008Article
- Activation of extracellular signaling regulated kinase in natural killer cells and monocytes following IL-2 stimulation in vitro and in patients undergoing IL-2 immunotherapy: analysis via dual parameter flow-cytometric assay.Cancer immunology, immunotherapy : CII · 2008Article
- Article
- Instant effect of soluble antigen on effector T cells in peripheral immune organs during immunotherapy of autoimmune encephalomyelitis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Quantitative single cell determination of ERK phosphorylation and regulation in relapsed and refractory primary acute myeloid leukemia.Leukemia · 2005Article
- A role for the Tec family kinase ITK in regulating SEB-induced interleukin-2 production in vivo via c-jun phosphorylation.BMC immunology · 2005Article
- Competition for self ligands restrains homeostatic proliferation of naive CD4 T cells.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Flow cytometry was used to study signaling events in individual CD4 T cells after antigen recognition in the body. Phosphorylation of c-jun and p38 mitogen-activated protein kinase was detected within minutes in all antigen-specific CD4 T cells in secondary lymphoid tissues after injection of peptide antigen into the bloodstream. The remarkable rapidity of this response correlated with the finding that most naive T cells are in constant contact with dendritic antigen-presenting cells. Contrary to predictions from in vitro experiments, antigen-induced c-jun and p38 mitogen-activated protein kinase phosphorylation did not depend on CD28 signals and was insensitive to inhibition by cyclosporin A. Our results highlight the efficiency of the in vivo immune response and underscore the need to verify which signaling pathways identified in vitro actually operate under physiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.