ArticleProceedings of the National Academy of Sciences of the United States of America2001
A src family tyrosine kinase inhibits neurotransmitter release from neuronal cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- Tyrosine kinases: multifaceted receptors at the intersection of several neurodegenerative disease-associated processes.Frontiers in dementia · 2024Review
- Mining the transcriptome of target tissues of autoimmune and degenerative pancreatic β-cell and brain diseases to discover therapies.iScience · 2022Article
- The tyrosine kinase v-Src modifies cytotoxicities of anticancer drugs targeting cell division.Journal of cellular and molecular medicine · 2021Article
- Inhibition of non-receptor tyrosine kinase Src induces phosphoserine 256-independent aquaporin-2 membrane accumulation.The Journal of physiology · 2019Article
- The tyrosine kinase v-Src causes mitotic slippage by phosphorylating an inhibitory tyrosine residue of Cdk1.The Journal of biological chemistry · 2018Article
- v-Src-driven transformation is due to chromosome abnormalities but not Src-mediated growth signaling.Scientific reports · 2018Article
- Tyrosine phosphorylation of Munc18-1 inhibits synaptic transmission by preventing SNARE assembly.The EMBO journal · 2018Article
- Application of High-Throughput Assays to Examine Phospho-Modulation of the Late Steps of Regulated Exocytosis.High-throughput · 2017Article
- N1-Src Kinase Is Required for Primary Neurogenesis inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Protective role for lipid modifications of Src-family kinases against chromosome missegregation.Scientific reports · 2016Article
- The N2-Src neuronal splice variant of C-Src has altered SH3 domain ligand specificity and a higher constitutive activity than N1-Src.FEBS letters · 2015Article
- Src family kinases promote silencing of ATR-Chk1 signaling in termination of DNA damage checkpoint.The Journal of biological chemistry · 2014Article
- Tyrosine 402 phosphorylation of Pyk2 is involved in ionomycin-induced neurotransmitter release.PloS one · 2014Article
- Myosin VI small insert isoform maintains exocytosis by tethering secretory granules to the cortical actin.The Journal of cell biology · 2013Article
- Constitutive activity of the A2A adenosine receptor and compartmentalised cyclic AMP signalling fine-tune noradrenaline release.Purinergic signalling · 2012Article
- Paxillin and phospholipase D interact to regulate actin-based processes in Dictyostelium discoideum.Eukaryotic cell · 2011Article
- Lyn kinase regulates mesolimbic dopamine release: implication for alcohol reward.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Genetic variation influences glutamate concentrations in brains of patients with multiple sclerosis.Brain : a journal of neurology · 2010Article
- Integrin signaling switches the cytoskeletal and exocytic machinery that drives neuritogenesis.Developmental cell · 2010Article
- Contribution of intracellular Ca2+ concentration and protein dephosphorylation to the induction of dopamine release from PC12 cells by the green odor compound hexanal.Cellular and molecular neurobiology · 2010Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tyrosine kinases are expressed in many tissues, particularly in the central nervous system, and regulate various cellular functions. We report here that a src family tyrosine kinase-specific inhibitor, PP2, enhances neurotransmitter release from PC12 cells and primary cultured neurons. PP2 enhances only Ca(2+)-dependent release; it does not affect basal release. These effects result from an enhancement of vesicular exocytosis and not from the reuptake or refilling of neurotransmitters because Ca(2+)-dependent secretion of an exogenously expressed reporter protein, the human growth hormone (hGH), is also enhanced by PP2. Overexpression of constitutive active v-src, but not of a kinase-inactive mutant, suppressed Ca(2+)-dependent release. In PP2-treated cells, Pyk2, paxillin, and some other proteins showed a decrease in tyrosine phosphorylation, and the enhancement of tyrosine phosphorylation of these proteins in response to Ca(2+) influx was also reduced. Electron and fluorescence microscopy showed that PP2 treatment induced morphological change and decreased phalloidin reactivity at the filopodium-like structures on the processes of PC12 cells. Interestingly, inhibition of actin polymerization with cytochalasin D and latrunculin A enhanced Ca(2+)-dependent, but not basal, release. It is possible that a src family tyrosine kinase, through the regulation of actin dynamics, has an inhibitory function to regulate neurotransmitter release.
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