ArticleThe Biochemical journal2001
Molecular events involved in up-regulating human Na+-independent neutral amino acid transporter LAT1 during T-cell activation.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 68 citations in OpenAlex.
- The human LAT1-4F2hc (SLC7A5-SLC3A2) transporter complex: Physiological and pathophysiological implications.Basic & clinical pharmacology & toxicology · 2023Review
- Tryptophan and its metabolites in normal physiology and cancer etiology.The FEBS journal · 2023Review
- Comparison of Experimental Strategies to Study l-Type Amino Acid Transporter 1 (LAT1) Utilization by Ligands.Molecules (Basel, Switzerland) · 2021Article
- Design and Development of Nanomaterial-Based Drug Carriers to Overcome the Blood-Brain Barrier by Using Different Transport Mechanisms.International journal of molecular sciences · 2021Review
- Single-cell analysis by mass cytometry reveals metabolic states of early-activated CD8Immunity · 2021Article
- Protein kinase C activation upregulates human L-type amino acid transporter 2 function.The journal of physiological sciences : JPS · 2021Article
- Towards antigen-specific Tregs for type 1 diabetes: Construction and functional assessment of pancreatic endocrine marker, HPi2-based chimeric antigen receptor.Cellular immunology · 2020Article
- Targeting of Perforin Inhibitor into the Brain Parenchyma Via a Prodrug Approach Can Decrease Oxidative Stress and Neuroinflammation and Improve Cell Survival.Molecular neurobiology · 2020Article
- Metabolite Transporters as Regulators of Immunity.Metabolites · 2020Review
- Metabolism of immune cells in cancer.Nature reviews. Cancer · 2020Review
- Hemocompatible LAT1-inhibitor can induce apoptosis in cancer cells without affecting brain amino acid homeostasis.Apoptosis : an international journal on programmed cell death · 2020Article
- Rigorous sampling of docking poses unveils binding hypothesis for the halogenated ligands of L-type Amino acid Transporter 1 (LAT1).Scientific reports · 2019Article
- Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc.Nature structural & molecular biology · 2019Article
- Article
- Amino-acid transporters in T-cell activation and differentiation.Cell death & disease · 2017Review
- Aryl hydrocarbon receptor (AHR) regulation of L-Type Amino Acid Transporter 1 (LAT-1) expression in MCF-7 and MDA-MB-231 breast cancer cells.Biochemical pharmacology · 2016Article
- Blood-brain barrier transport machineries and targeted therapy of brain diseases.BioImpacts : BI · 2016Review
- L-type amino acid transport and cancer: targeting the mTORC1 pathway to inhibit neoplasia.American journal of cancer research · 2015Review
- Membrane transporters for the special amino acid glutamine: structure/function relationships and relevance to human health.Frontiers in chemistry · 2014Review
- Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the regulation of system-L amino acid transporter (LAT1) during T-cell activation. In quiescent T-cells, L-leucine transport is mediated mainly by the system-L amino acid transport system and is increased significantly during T-cell activation by PMA and ionomycin. In quiescent T-cells, the LAT1 protein was heterocomplexed with 4F2 heavy chain (4F2hc) in the plasma membrane. During T-cell activation, the amounts of 4F2hc and LAT1 heterocomplex were significantly elevated compared with those in quiescent T-cells. In addition, by Northern-blot analysis, these increments were found to be due to elevated levels of LAT1 and 4F2hc mRNA. Transient expression of constructs comprising various LAT1 gene promoter fragments, which contained all three of the GC boxes, was sufficient for promoting luciferase expression in Jurkat T-cells, but the promoter of the LAT1 gene did not respond to PMA and ionomycin. Similar observations were observed in the human 4F2hc gene promoter. In nuclear run-on assay, the LAT1 and 4F2hc genes were actively transcribed even in quiescent T-cells, but the low levels of both transcripts were shown to be the result of a block to transcription elongation within the exon 1 intron 1 regions. These findings indicated that a removal of the block to mRNA elongation stimulates the induction of system-L amino acid transporter gene transcripts (LAT1 and 4F2hc) in activated T-cells.
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