ArticleMolecular and cellular biology2001
Functional analysis of the cyclin-dependent kinase inhibitor Pho81 identifies a novel inhibitory domain.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Mining key genes associated with phosphorus deficiency through genome-wide identification and characterization of cucumber SPX family genes.BMC plant biology · 2024Article
- A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy.Nature cell biology · 2024Article
- Inositol pyrophosphate dynamics reveals control of the yeast phosphate starvation program through 1,5-IPeLife · 2023Article
- The nutrient-responsive CDK Pho85 primes the Sch9 kinase for its activation by TORC1.PLoS genetics · 2023Article
- IPmBio · 2020Article
- Phosphate Homeostasis - A Vital Metabolic Equilibrium Maintained Through the INPHORS Signaling Pathway.Frontiers in microbiology · 2020Review
- Rice SPX1 and SPX2 inhibit phosphate starvation responses through interacting with PHR2 in a phosphate-dependent manner.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Defects in phosphate acquisition and storage influence virulence of Cryptococcus neoformans.Infection and immunity · 2014Article
- Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae.Genetics · 2012Article
- Phosphate sensing.Advances in chronic kidney disease · 2011Review
- Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae.Current genetics · 2010Review
- Tracking lineages of single cells in lines using a microfluidic device.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Haemophilus ducreyi LspA proteins are tyrosine phosphorylated by macrophage-encoded protein tyrosine kinases.Infection and immunity · 2008Article
- Molecular basis of cyclin-CDK-CKI regulation by reversible binding of an inositol pyrophosphate.Nature chemical biology · 2008Article
- Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway.Molecular cell · 2007Article
- Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates.Science (New York, N.Y.) · 2007Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- Yeast Gcn4p stabilization is initiated by the dissociation of the nuclear Pho85p/Pcl5p complex.Molecular biology of the cell · 2006Article
- Rim15 and the crossroads of nutrient signalling pathways in Saccharomyces cerevisiae.Cell division · 2006Article
- Disruption of histone deacetylase gene RPD3 accelerates PHO5 activation kinetics through inappropriate Pho84p recycling.Eukaryotic cell · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes important for survival. A phosphate-responsive signal transduction pathway mediates this response by controlling the activity of the transcription factor Pho4. Three components of this signal transduction pathway resemble those used to regulate the eukaryotic cell cycle: a cyclin-dependent kinase (CDK), Pho85; a cyclin, Pho80; and a CDK inhibitor (CKI), Pho81. Pho81 forms a stable complex with Pho80-Pho85 under both high- and low-phosphate conditions, but it only inhibits the kinase when cells are starved for phosphate. Pho81 contains six tandem repeats of the ankyrin consensus domain homologous to the INK4 family of mammalian CKIs. INK4 proteins inhibit kinase activity through an interaction of the ankyrin repeats and the CDK subunits. Surprisingly, we find that a region of Pho81 containing 80 amino acids C terminal to the ankyrin repeats is necessary and sufficient for Pho81's CKI function. The ankyrin repeats of Pho81 appear to have no significant role in Pho81 inhibition. Our results suggest that Pho81 inhibits Pho80-Pho85 with a novel motif.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.