ArticleMolecular and cellular biology2001
Molecular genetic dissection of TAF25, an essential yeast gene encoding a subunit shared by TFIID and SAGA multiprotein transcription factors.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- TAF9b (formerly TAF9L) is a bona fide TAF that has unique and overlapping roles with TAF9.Molecular and cellular biology · 2005Article
- The nuclear import of TAF10 is regulated by one of its three histone fold domain-containing interaction partners.Molecular and cellular biology · 2005Article
- TAF10 (TAF(II)30) is necessary for TFIID stability and early embryogenesis in mice.Molecular and cellular biology · 2003Article
- Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP).Nucleic acids research · 2003Article
- Molecular characterization of Saccharomyces cerevisiae TFIID.Molecular and cellular biology · 2002Article
- Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry.Molecular and cellular biology · 2002Article
- Distinct mutations in yeast TAF(II)25 differentially affect the composition of TFIID and SAGA complexes as well as global gene expression patterns.Molecular and cellular biology · 2002Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have performed a systematic structure-function analysis of Saccharomyces cerevisiae TAF25, an evolutionarily conserved, single-copy essential gene which encodes the 206-amino-acid TAF25p protein. TAF25p is an integral subunit of both the 15-subunit general transcription factor TFIID and the multisubunit, chromatin-acetylating transcriptional coactivator SAGA. We used hydroxylamine mutagenesis, targeted deletion, alanine-scanning mutagenesis, high-copy suppression methods, and two-hybrid screening to dissect TAF25. Temperature-sensitive mutant strains generated were used for coimmunoprecipitation and transcription analyses to define the in vivo functions of TAF25p. The results of these analyses show that TAF25p is comprised of multiple mutable elements which contribute importantly to RNA polymerase II-mediated mRNA gene transcription.
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