ArticleMolecular and cellular biology2001
Polycomb group repression reduces DNA accessibility.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 71 citations in OpenAlex.
- Chromatin folding by the Polycomb group proteins and its elusive role in epigenetic repression.The FEBS journal · 2026Review
- Isha is a su(Hw) mRNA-binding protein required for gypsy insulator function.G3 (Bethesda, Md.) · 2022Article
- Molecular Lessons from theGenetics · 2020Review
- DifferentiatingeLife · 2020Article
- Dynamic Competition of Polycomb and Trithorax in Transcriptional Programming.Annual review of biochemistry · 2020Review
- Identification of genomic enhancers through spatial integration of single-cell transcriptomics and epigenomics.Molecular systems biology · 2020Article
- Polycomb repressive complex 1 shapes the nucleosome landscape but not accessibility at target genes.Genome research · 2018Article
- Review
- On the Mechanism of Gene Silencing in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2015Article
- Heterochromatic gene silencing by activator interference and a transcription elongation barrier.The Journal of biological chemistry · 2013Article
- Messenger RNA is a functional component of a chromatin insulator complex.EMBO reports · 2013Article
- The border between the ultrabithorax and abdominal-A regulatory domains in the Drosophila bithorax complex.Genetics · 2013Article
- Progressive polycomb assembly on H3K27me3 compartments generates polycomb bodies with developmentally regulated motion.PLoS genetics · 2012Article
- Genome-wide analysis of the binding of the Hox protein Ultrabithorax and the Hox cofactor Homothorax in Drosophila.PloS one · 2011Article
- Accessibility of the Drosophila genome discriminates PcG repression, H4K16 acetylation and replication timing.Nature structural & molecular biology · 2010Article
- Ring1B compacts chromatin structure and represses gene expression independent of histone ubiquitination.Molecular cell · 2010Article
- Characterization of new regulatory elements within the Drosophila bithorax complex.Nucleic acids research · 2008Article
- Inferring causal relationships among different histone modifications and gene expression.Genome research · 2008Article
- Replacement of a Drosophila Polycomb response element core, and in situ analysis of its DNA motifs.Molecular genetics and genomics : MGG · 2008Article
- MicroRNAs in the Drosophila bithorax complex.Genes & development · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Polycomb group proteins are responsible for long-term repression of a number of genes in Drosophila melanogaster, including the homeotic genes of the bithorax complex. The Polycomb protein is thought to alter the chromatin structure of its target genes, but there has been little direct evidence for this model. In this study, the chromatin structure of the bithorax complex was probed with three separate assays for DNA accessibility: (i) activation of polymerase II (Pol II) transcription by Gal4, (ii) transcription by the bacteriophage T7 RNA polymerase (T7RNAP), and (iii) FLP-mediated site-specific recombination. All three processes are restricted or blocked in Polycomb-repressed segments. In contrast, control test sites outside of the bithorax complex permitted Gal4, T7RNAP, and FLP activities throughout the embryo. Several P insertions in the bithorax complex were tested, providing evidence that the Polycomb-induced effect is widespread over target genes. This accessibility effect is similar to that seen for SIR silencing in Saccharomyces cerevisiae. In contrast to SIR silencing, however, episomes excised from Polycomb-repressed chromosomal sites do not show an altered superhelix density.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.