ArticleJournal of virology2001
Identification of a central DNA flap in feline immunodeficiency virus.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- The fourth central polypurine tract guides the synthesis of prototype foamy virus plus-strand DNA.Virus genes · 2017Article
- The importance of becoming double-stranded: Innate immunity and the kinetic model of HIV-1 central plus strand synthesis.Virology · 2013Review
- Assessment of Integration-defective HIV-1 and EIAV Vectors In Vitro and In Vivo.Molecular therapy. Nucleic acids · 2012Article
- Residual HIV-1 DNA Flap-independent nuclear import of cPPT/CTS double mutant viruses does not support spreading infection.Retrovirology · 2011Article
- Review
- The HIV-1 central polypurine tract functions as a second line of defense against APOBEC3G/F.Journal of virology · 2010Article
- Prostaglandin pathway gene therapy for sustained reduction of intraocular pressure.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Conserved footprints of APOBEC3G on Hypermutated human immunodeficiency virus type 1 and human endogenous retrovirus HERV-K(HML2) sequences.Journal of virology · 2008Article
- Human gene therapy vectors derived from feline lentiviruses.Veterinary immunology and immunopathology · 2008Review
- Evolution of feline immunodeficiency virus in Felidae: implications for human health and wildlife ecology.Veterinary immunology and immunopathology · 2008Review
- Genomic organization, sequence divergence, and recombination of feline immunodeficiency virus from lions in the wild.BMC genomics · 2008Article
- Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivo dendritic cells and T lymphocytes.Genetic vaccines and therapy · 2007Article
- Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Nuclear import defect of human immunodeficiency virus type 1 DNA flap mutants is not dependent on the viral strain or target cell type.Journal of virology · 2006Article
- Functional central polypurine tract provides downstream protection of the human immunodeficiency virus type 1 genome from editing by APOBEC3G and APOBEC3B.Journal of virology · 2006Article
- Identification and characterization of an exogenous retrovirus from atlantic salmon swim bladder sarcomas.Journal of virology · 2006Article
- Feline lentivirus evolution in cross-species infection reveals extensive G-to-A mutation and selection on key residues in the viral polymerase.Journal of virology · 2006Article
- Effect of polypurine tract (PPT) mutations on human immunodeficiency virus type 1 replication: a virus with a completely randomized PPT retains low infectivity.Journal of virology · 2005Article
- LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes.Journal of virology · 2004Article
- Mapping the encapsidation determinants of feline immunodeficiency virus.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A duplication of the polypurine tract (PPT) at the center of the human immunodeficiency virus type 1 (HIV-1) genome (the cPPT) has been shown to prime a separate plus-strand initiation and to result in a plus-strand displacement (DNA flap) that plays a role in nuclear import of the viral preintegration complex. Feline immunodeficiency virus (FIV) is a lentivirus that infects nondividing cells, causes progressive CD4(+) T-cell depletion, and has been used as a substrate for lentiviral vectors. However, the PPT sequence is not duplicated elsewhere in the FIV genome and a central plus-strand initiation or strand displacement has not been identified. Using Southern blotting of S1 nuclease-digested FIV preintegration complexes isolated from infected cells, we detected a single-strand discontinuity at the approximate center of the reverse-transcribed genome. Primer extension analyses assigned the gap to the plus strand, and mapped the 5' terminus of the downstream (D+) segment to a guanine residue in a purine-rich tract in pol (AAAAGAAGAGGTAGGA). RACE experiments then mapped the 3' terminus of the upstream plus (U+)-strand segment to a T nucleotide located 88 nucleotides downstream of the D+ strand 5' terminus, thereby identifying the extent of D+ strand displacement and the central termination sequence of this virus. Unlike HIV, the FIV cPPT is significantly divergent in sequence from its 3' counterpart (AAAAAAGAAAAAAGGGTGG) and contains one and in some cases two pyrimidines. An invariant thymidine located -2 to the D+ strand origin is neither required nor optimal for codon usage at this position. Although the mapped cPPTs of FIV and HIV-1 act in cis, they encode homologous amino acids in integrase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.