ArticleJournal of virology2001
Interactions and nuclear import of the N and P proteins of sonchus yellow net virus, a plant nucleorhabdovirus.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 78 citations in OpenAlex.
- Identification, molecular characterization and phylogenetic analysis of a novel nucleorhabdovirus infecting Paris polyphylla var. yunnanensis.Scientific reports · 2023Article
- A novel Actinidia cytorhabdovirus characterized using genomic and viral protein interaction features.Molecular plant pathology · 2021Article
- Contribution of yeast models to virus research.Applied microbiology and biotechnology · 2021Review
- Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and InteractsFrontiers in microbiology · 2020Article
- The Matrix Protein of a Plant Rhabdovirus Mediates Superinfection Exclusion by Inhibiting Viral Transcription.Journal of virology · 2019Article
- A cytorhabdovirus phosphoprotein forms mobile inclusions trafficked on the actin/ER network for viral RNA synthesis.Journal of experimental botany · 2019Article
- Specificity of Plant Rhabdovirus Cell-to-Cell Movement.Journal of virology · 2019Article
- Host Range and Population Survey of Spodoptera frugiperda Rhabdovirus.Journal of virology · 2019Article
- Genomic characterization, phylogenetic position and in situ localization of a novel putative mononegavirus in Lepeophtheirus salmonis.Archives of virology · 2019Article
- Development of leafhopper cell culture to trace the early infection process of a nucleorhabdovirus, rice yellow stunt virus, in insect vector cells.Virology journal · 2018Article
- Article
- PHYTOCHROME INTERACTING FACTOR1 interactions leading to the completion or prolongation of seed germination.Plant signaling & behavior · 2018Article
- Construction of a Sonchus Yellow Net Virus minireplicon: a step toward reverse genetic analysis of plant negative-strand RNA viruses.Journal of virology · 2013Article
- Orchid fleck virus structural proteins N and P form intranuclear viroplasm-like structures in the absence of viral infection.Journal of virology · 2013Article
- The Nucleocapsid Protein of Potato Yellow dwarf Virus: Protein Interactions and Nuclear Import Mediated by a Non-Canonical Nuclear Localization Signal.Frontiers in plant science · 2012Article
- Zinc finger nuclease and homing endonuclease-mediated assembly of multigene plant transformation vectors.Plant physiology · 2012Article
- RNA splicing in a new rhabdovirus from Culex mosquitoes.Journal of virology · 2011Article
- Rice black-streaked dwarf virus P6 self-interacts to form punctate, viroplasm-like structures in the cytoplasm and recruits viroplasm-associated protein P9-1.Virology journal · 2011Article
- An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus.Virology · 2010Article
- Distinctive interactions of the Arabidopsis homolog of the 30 kD subunit of the cleavage and polyadenylation specificity factor (AtCPSF30) with other polyadenylation factor subunits.BMC cell biology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized the interaction and nuclear localization of the nucleocapsid (N) protein and phosphoprotein (P) of sonchus yellow net nucleorhabdovirus. Expression studies with plant and yeast cells revealed that both N and P are capable of independent nuclear import. Site-specific mutagenesis and deletion analyses demonstrated that N contains a carboxy-terminal bipartite nuclear localization signal (NLS) located between amino acids 465 and 481 and that P contains a karyophillic region between amino acids 40 and 124. The N NLS was fully capable of functioning outside of the context of the N protein and was able to direct the nuclear import of a synthetic protein fusion consisting of green fluorescent protein fused to glutathione S-transferase (GST). Expression and mapping studies suggested that the karyophillic domain in P is located within the N-binding domain. Coexpression of N and P drastically affected their localization patterns relative to those of individually expressed proteins and resulted in a shift of both proteins to a subnuclear region. Yeast two-hybrid and GST pulldown experiments verified the N-P and P-P interactions, and deletion analyses have identified the N and P interacting domains. N NLS mutants were not transported to the nucleus by import-competent P, presumably because N binding masks the P NLS. Taken together, our results support a model for independent entry of N and P into the nucleus followed by associations that mediate subnuclear localization.
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